Vaccinia Virus and Poxvirology

Methods and Protocols

Editors:

ISBN: 978-1-61779-875-7 (Print) 978-1-61779-876-4 (Online)

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiii

  2. No Access

    Book Protocol

    Pages 1-22

    Working Safely with Vaccinia Virus: Laboratory Technique and Review of Published Cases of Accidental Laboratory Infections

  3. No Access

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    Pages 23-35

    In-Fusion® Cloning with Vaccinia Virus DNA Polymerase

  4. No Access

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    Pages 37-57

    Genetic Manipulation of Poxviruses Using Bacterial Artificial Chromosome Recombineering

  5. No Access

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    Pages 59-92

    Easy and Efficient Protocols for Working with Recombinant Vaccinia Virus MVA

  6. No Access

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    Pages 93-111

    Isolation of Recombinant MVA Using F13L Selection

  7. No Access

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    Pages 113-121

    Screening for Vaccinia Virus Egress Inhibitors: Separation of IMV, IEV, and EEV

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    Pages 123-133

    Imaging of Vaccinia Virus Entry into HeLa Cells

  9. No Access

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    Pages 135-146

    New Method for the Assessment of Molluscum Contagiosum Virus Infectivity

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    Pages 147-159

    An Intradermal Model for Vaccinia Virus Pathogenesis in Mice

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    Pages 161-176

    Measurements of Vaccinia Virus Dissemination Using Whole Body Imaging: Approaches for Predicting of Lethality in Challenge Models and Testing of Vaccines and Antiviral Treatments

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    Pages 177-198

    Mousepox, A Small Animal Model of Smallpox

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    Pages 199-218

    Analyzing CD8 T Cells in Mouse Models of Poxvirus Infection

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    Pages 219-232

    Generation and Characterization of Monoclonal Antibodies Specific for Vaccinia Virus

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    Pages 233-258

    Bioinformatics for Analysis of Poxvirus Genomes

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    Pages 259-271

    Antigen Presentation Assays to Investigate Uncharacterized Immunoregulatory Genes

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    Pages 273-288

    Characterization of Poxvirus-Encoded Proteins that Regulate Innate Immune Signaling Pathways

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    Pages 289-303

    Application of Quartz Crystal Microbalance with Dissipation Monitoring Technology for Studying Interactions of Poxviral Proteins with Their Ligands

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    Pages 305-326

    Central Nervous System Distribution of the Poxviral Proteins After Intranasal Administration of Proteins and Titering of Vaccinia Virus in the Brain After Intracranial Administration

  20. Back Matter

    Pages 327-332