Kidney Development

Methods and Protocols

Editors:

ISBN: 978-1-61779-850-4 (Print) 978-1-61779-851-1 (Online)

Table of contents (36 protocols)

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  1. Front Matter

    Pages i-xiv

  2. Organ Culture, Tissue Manipulation, and Live Imaging

    1. Front Matter

      Pages 1-1

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      Pages 3-11

      Dissecting and Culturing and Imaging the Mouse Urogenital System

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      Pages 13-21

      In Vitro Culture of Embryonic Kidney Rudiments and Isolated Ureteric Buds

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      Pages 23-30

      In Vitro Induction of Nephrogenesis in Mouse Metanephric Mesenchyme with Lithium Introduction and Ureteric Bud Recombination

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      Pages 31-43

      Live Imaging of the Developing Mouse Mesonephros

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      Pages 45-53

      Organotypic Culture of the Urogenital Tract

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      Pages 55-70

      Live Imaging Kidney Development in Zebrafish

  3. Analyzing Ureteric Bud Branching and Nephrogenesis

    1. Front Matter

      Pages 71-71

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      Pages 73-86

      Analysis of 3D Branching Pattern: Hematoxylin and Eosin Method

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      Pages 87-93

      Three-Dimensional Imaging of Fetal Mouse Kidneys

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      Pages 95-107

      Analysis of Native Kidney Structures in Three Dimensions

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      Pages 109-119

      Estimating Nephron Number in the Developing Kidney Using the Physical Disector/Fractionator Combination

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      Pages 121-132

      An Immunofluorescence Method to Analyze the Proliferation Status of Individual Nephron Segments in the Xenopus Pronephric Kidney

  4. Renal Primary Cell Cultures and Cell Lines

    1. Front Matter

      Pages 133-133

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      Pages 135-146

      Dissociation of Embryonic Kidney Followed by Re-aggregation as a Method for Chimeric Analysis

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      Pages 147-155

      Analysis of Migration in Primary Ureteric Bud Epithelial Cells

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      Pages 157-163

      Investigating Primary Cilia in Cultured Metanephric Mesenchymal Cells

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      Pages 165-171

      Making Immortalized Cell Lines from Embryonic Mouse Kidney

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      Pages 173-182

      Engineered Tissues to Quantify Collective Cell Migration During Morphogenesis

  5. Detecting Gene/Protein Expression and Signaling

    1. Front Matter

      Pages 183-183

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      Pages 185-201

      Access and Use of the GUDMAP Database of Genitourinary Development

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      Pages 203-210

      Isolation of High Quality RNA from Embryonic Kidney and Cells

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      Pages 211-221

      Laser Capture

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      Pages 223-239

      Use of In Situ Hybridization to Examine Gene Expression in the Embryonic, Neonatal, and Adult Urogenital System

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