Retinal Development

Methods and Protocols

Editors:

ISBN: 978-1-61779-847-4 (Print) 978-1-61779-848-1 (Online)

Table of contents (25 protocols)

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  1. Front Matter

    Pages i-xiv

  2. Manipulation of Gene Expression in vivo

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-15

      Conditional Control of Gene Expression in the Mouse Retina

    3. No Access

      Book Protocol

      Pages 17-39

      Generation of Transgenic Xenopus Using Restriction Enzyme-Mediated Integration

    4. No Access

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      Pages 41-51

      In Vivo Functional Analysis of Transcription Factor: Response Element Interaction Using Transgenic Xenopus laevis

    5. No Access

      Book Protocol

      Pages 53-69

      Subretinal Delivery and Electroporation in Pigmented and Nonpigmented Adult Mouse Eyes

    6. No Access

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      Pages 71-89

      In Ovo Eye Electroporation

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      Pages 91-111

      Targeted Microinjection of Synthetic mRNAs to Alter Retina Gene Expression in Xenopus Embryos

  3. Tracing Cell Fate

    1. Front Matter

      Pages 113-113

    2. No Access

      Book Protocol

      Pages 115-127

      Testing Retina Fate Commitment in Xenopus by Blastomere Deletion, Transplantation, and Explant Culture

    3. No Access

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      Pages 129-140

      Application of Cre-loxP Recombination for Lineage Tracing of Adult Zebrafish Retinal Stem Cells

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      Pages 141-152

      Fate Tracing of neurogenin2-Expressing Cells in the Mouse Retina Using CreER™: LacZ

  4. In vitro Systems

    1. Front Matter

      Pages 153-153

    2. No Access

      Book Protocol

      Pages 155-165

      In Vitro Explant Culture and Related Protocols for the Study of Mouse Retinal Development

    3. No Access

      Book Protocol

      Pages 167-181

      In Vitro Biochemical Assays to Monitor Rhodopsin Function

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      Pages 183-192

      Transfection of Primary Embryonic Chicken Retinal Cells Using Cationic Lipid

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      Book Protocol

      Pages 193-199

      Production of High-Titer RCAS Retrovirus

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      Pages 201-209

      Chick Retinal Pigment Epithelium Transdifferentiation Assay for Proneural Activities

  5. Regeneration/Stem Cells

    1. Front Matter

      Pages 211-211

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      Book Protocol

      Pages 213-227

      Studying the Generation of Regenerated Retinal Neuron from Müller Glia in the Mouse Eye

    3. No Access

      Book Protocol

      Pages 229-246

      Production and Transplantation of Retinal Cells from Human and Mouse Embryonic Stem Cells

    4. No Access

      Book Protocol

      Pages 247-254

      Light-Induced Photoreceptor Degeneration in the Retina of the Zebrafish

    5. No Access

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      Pages 255-261

      Microarray-Based Gene Profiling Analysis of Müller Glia-Derived Retinal Stem Cells in Light-Damaged Retinas from Adult Zebrafish

  6. Function/Imaging

    1. Front Matter

      Pages 263-263

    2. No Access

      Book Protocol

      Pages 265-276

      Measuring Rodent Electroretinograms to Assess Retinal Function

    3. No Access

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      Pages 277-285

      Functional Imaging of Retinal Photoreceptors and Inner Neurons Using Stimulus-Evoked Intrinsic Optical Signals

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