Immunogenetics

Methods and Applications in Clinical Practice

Editors:

ISBN: 978-1-61779-841-2 (Print) 978-1-61779-842-9 (Online)

Table of contents (34 protocols)

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  1. Front Matter

    Pages i-xvi

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    Pages 1-7

    The Major Histocompatibility Complex: A Paradigm for Studies of the Human Genome

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    Pages 9-25

    HLA Typing by SSO and SSP Methods

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    Pages 27-46

    Methods for Diagnostic HLA Typing in Disease Association and Drug Hypersensitivity

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    Pages 47-65

    HLA Typing Using Bead-Based Methods

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    Pages 67-86

    HLA Typing by Direct DNA Sequencing

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    Pages 87-121

    Data Analysis of HLA Sequencing Using Assign-SBT v3.6+ from Conexio

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    Pages 123-142

    Simple Methods for the Detection of HLA-G Variants in Coding and Non-coding Regions

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    Pages 143-158

    Molecular Typing of HLA-E

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    Pages 159-171

    Molecular Analysis of Complement Component C4 Gene Copy Number

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    Pages 173-182

    Genotyping of Single Nucleotide Polymorphisms by 5′ Nuclease Allelic Discrimination

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    Pages 183-195

    High Resolution MICA Genotyping by Sequence-Based Typing (SBT)

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    Pages 197-213

    Standard Methods for the Management of Immunogenetic Data

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    Pages 215-244

    Analytical Methods for Immunogenetic Population Data

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    Pages 245-266

    Analytical Methods for Disease Association Studies with Immunogenetic Data

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    Pages 267-277

    Impact of HLA Matching and HLA Antibodies in Organ Transplantation: A Collaborative Transplant Study View

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    Pages 279-288

    Screening for Antibodies Against MICA by Luminex Flow Cytometry

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    Pages 289-308

    HLA Antibody Detection and Characterization by Solid Phase Immunoassays: Methods and Pitfalls

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    Pages 309-337

    Detection and Characterisation of Alloreactive T Cells

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    Pages 339-349

    Detection of Allo-HLA Cross-Reactivity by Virus-specific Memory T-Cell Clones Using Single HLA-Transfected K562 Cells

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    Pages 351-357

    Separation and Cryopreservation of Lymphocytes from Spleen and Lymph Node

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