Somatic Stem Cells

Methods and Protocols

Editors:

ISBN: 978-1-61779-814-6 (Print) 978-1-61779-815-3 (Online)

Table of contents (33 protocols)

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  1. Front Matter

    Pages i-xvi

  2. Introduction

    1. Front Matter

      Pages 1-1

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      Pages 3-26

      Current Thoughts on the Therapeutic Potential of Stem Cell

  3. Stem Cell Study in Lower Organism

    1. Front Matter

      Pages 27-27

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      Pages 29-37

      A Unique FACS Method to Isolate Stem Cells in Planarian

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      Pages 39-46

      Identification of Neural Stem Cells in the Drosophila Larval Brain

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      Pages 47-69

      Generation and Staining of Intestinal Stem Cell Lineage in Adult Midgut

  4. Stem Cell Study in Murine and Human Model

    1. Front Matter

      Pages 71-71

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      Pages 73-88

      Developing a Quantitative In Vivo Tissue Reconstitution Assay to Assess the Relative Potency of Candidate Populations of Mouse Oesophageal Epithelial Cells

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      Pages 89-107

      Identification, Isolation, and Culture of Intestinal Epithelial Stem Cells from Murine Intestine

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      Pages 109-122

      Isolation and Characterization of Distal Lung Progenitor Cells

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      Pages 123-133

      Transplantation of Mouse Fetal Liver Cells for Analyzing the Function of Hematopoietic Stem and Progenitor Cells

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      Pages 135-146

      Convenient and Efficient Enrichment of the CD133+ Liver Cells from Rat Fetal Liver as a Source of Liver Stem/Progenitor Cells

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      Pages 147-164

      Assessing the Potential Clinical Utility of Transplantations of Neural and Mesenchymal Stem Cells for Treating Neurodegenerative Diseases

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      Pages 165-178

      Functional Identification of Neural Stem Cell-Derived Oligodendrocytes

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      Pages 179-193

      Stem/Progenitor Cells in Murine Mammary Gland: Isolation and Functional Characterization

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      Pages 195-201

      A Reporter Assay to Detect Transfer and Targeting of miRNAs in Stem Cell-Breast Cancer Co-cultures

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      Pages 203-267

      Isolation, Culture, and Osteogenic/Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells

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      Pages 269-278

      Obtaining Freshly Isolated and Cultured Mesenchymal Stem Cells from Human Adipose Tissue

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      Pages 279-290

      Collection, Processing, and Banking of Umbilical Cord Blood Stem Cells for Transplantation and Regenerative Medicine

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      Pages 291-313

      Generation of Functional Islets from Human Umbilical Cord and Placenta Derived Mesenchymal Stem Cells

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      Pages 315-326

      Isolation and Characterization of Human Prostate Stem/Progenitor Cells

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      Pages 327-338

      Isolation and Expansion of Adult Cardiac Stem/Progenitor Cells in the Form of Cardiospheres from Human Cardiac Biopsies and Murine Hearts

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      Pages 339-349

      Isolation and Differentiation of Human Cardiomyocyte Progenitor Cells into Cardiomyocytes

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