Plant Signalling Networks

Methods and Protocols

Editors:

ISBN: 978-1-61779-808-5 (Print) 978-1-61779-809-2 (Online)

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi

  2. No Access

    Book Protocol

    Pages 1-15

    Experimental Analysis of Receptor Kinase Phosphorylation

  3. No Access

    Book Protocol

    Pages 17-32

    Quantitative Measurement of Phosphopeptides and Proteins via Stable Isotope Labeling in Arabidopsis and Functional Phosphoproteomic Strategies

  4. No Access

    Book Protocol

    Pages 33-45

    Identification of O-linked β-d-N-acetylglucosamine-Modified Proteins from Arabidopsis

  5. No Access

    Book Protocol

    Pages 47-66

    Quantitative Analysis of Protein Phosphorylation Using Two-Dimensional Difference Gel Electrophoresis

  6. No Access

    Book Protocol

    Pages 67-82

    Quantitative Analysis of Plasma Membrane Proteome Using Two-Dimensional Difference Gel Electrophoresis

  7. No Access

    Book Protocol

    Pages 83-94

    Identification and Verification of Redox-Sensitive Proteins in Arabidopsis thaliana

  8. No Access

    Book Protocol

    Pages 95-106

    Small-Molecule Dissection of Brassinosteroid Signaling

  9. No Access

    Book Protocol

    Pages 107-116

    A Chemical Genetics Method to Uncover Small Molecules for Dissecting the Mechanism of ABA Responses in Arabidopsis Seed Germination

  10. No Access

    Book Protocol

    Pages 117-133

    Activation Tagging

  11. No Access

    Book Protocol

    Pages 135-144

    Rho GTPase Activity Analysis in Plant Cells

  12. No Access

    Book Protocol

    Pages 145-152

    Analysis of In Vivo ROP GTPase Activity at the Subcellular Level by Fluorescence Resonance Energy Transfer Microscopy

  13. No Access

    Book Protocol

    Pages 153-162

    In Vivo Ubiquitination Assay by Agroinfiltration

  14. No Access

    Book Protocol

    Pages 163-172

    In Vitro Protein Ubiquitination Assay

  15. No Access

    Book Protocol

    Pages 173-188

    Genome-Wide Identification of Transcription Factor-Binding Sites in Plants Using Chromatin Immunoprecipitation Followed by Microarray (ChIP-chip) or Sequencing (ChIP-seq)

  16. No Access

    Book Protocol

    Pages 189-194

    Smart Pooling of mRNA Samples for Efficient Transcript Profiling

  17. No Access

    Book Protocol

    Pages 195-206

    Transient Expression Assays for Quantifying Signaling Output

  18. No Access

    Book Protocol

    Pages 207-216

    Genome-Wide Profiling of Uncapped mRNA

  19. No Access

    Book Protocol

    Pages 217-227

    Computational Tools for Quantitative Analysis of Cell Growth Patterns and Morphogenesis in Actively Developing Plant Stem Cell Niches

  20. Back Matter

    Pages 229-230