Spectroscopic Methods of Analysis

Methods and Protocols

Editors:

ISBN: 978-1-61779-805-4 (Print) 978-1-61779-806-1 (Online)

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii

  2. No Access

    Book Protocol

    Pages 1-22

    Fluorescence Lifetime Imaging Comes of Age How to Do It and How to Interpret It

  3. No Access

    Book Protocol

    Pages 23-55

    Förster Resonance Energy Transfer and Trapping in Selected Systems: Analysis by Monte-Carlo Simulation

  4. No Access

    Book Protocol

    Pages 57-65

    Molecular Organization of Polyene Antibiotic Amphotericin B Studied by Means of Fluorescence Technique

  5. No Access

    Book Protocol

    Pages 67-84

    Spectroscopic Probes of RNA Structure and Dynamics

  6. No Access

    Book Protocol

    Pages 85-104

    Fluorescence Methods to Study DNA Translocation and Unwinding Kinetics by Nucleic Acid Motors

  7. No Access

    Book Protocol

    Pages 105-133

    Fluorescence Intensity, Anisotropy, and Transient Dynamic Quenching Stopped-Flow Kinetics

  8. No Access

    Book Protocol

    Pages 135-164

    Using Structure–Function Constraints in FRET Studies of Large Macromolecular Complexes

  9. No Access

    Book Protocol

    Pages 165-192

    Illuminating Allostery in Metal Sensing Transcriptional Regulators

  10. No Access

    Book Protocol

    Pages 193-216

    Fluorescence-Based Biosensors

  11. No Access

    Book Protocol

    Pages 217-229

    Metal-Enhanced Immunoassays

  12. No Access

    Book Protocol

    Pages 231-239

    Initial Stages of Angiosperm Greening Monitored by Low-Temperature Fluorescence Spectra and Fluorescence Lifetimes

  13. No Access

    Book Protocol

    Pages 241-251

    Activation of the Mammalian Cells by Using Light-Sensitive Ion Channels

  14. No Access

    Book Protocol

    Pages 253-262

    Detection of Specific Strains of Viable Bacterial Pathogens by Using RNA Bead Assays and Flow Cytometry with 2100 Bioanalyzer

  15. No Access

    Book Protocol

    Pages 263-269

    Photosynthetic Antenna Complex LHCII Studied with Novel Fluorescence Techniques

  16. No Access

    Book Protocol

    Pages 271-295

    Analysis of RNA Folding and Ribonucleoprotein Assembly by Single-Molecule Fluorescence Spectroscopy

  17. No Access

    Book Protocol

    Pages 297-310

    Single-Molecule Force Spectroscopy of Polycystic Kidney Disease Proteins

  18. No Access

    Book Protocol

    Pages 311-334

    Single Molecule Detection Approach to Muscle Study: Kinetics of a Single Cross-Bridge During Contraction of Muscle

  19. No Access

    Book Protocol

    Pages 335-356

    Single-Molecule Optical-Trapping Measurements with DNA Anchored to an Array of Gold Nanoposts

  20. No Access

    Book Protocol

    Pages 357-373

    Electro-optical Analysis of Macromolecular Structure and Dynamics

  21. No Access

    Book Protocol

    Pages 375-391

    Cryoradiolysis and Cryospectroscopy for Studies of Heme-Oxygen Intermediates in Cytochromes P450

  22. Back Matter

    Pages 393-397