Human Embryonic Stem Cells Handbook

Editors:

ISBN: 978-1-61779-793-4 (Print) 978-1-61779-794-1 (Online)

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi

  2. No Access

    Book Protocol

    Pages 1-12

    Establishment of New Lines of Human Embryonic Stem Cells: Evolution of the Methodology

  3. No Access

    Book Protocol

    Pages 13-32

    Human Embryonic Stem Cells Derived in Xeno-Free Conditions

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    Book Protocol

    Pages 33-51

    Procedures for Derivation and Characterisation of Human Embryonic Stem Cells from Odense, Denmark

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    Book Protocol

    Pages 53-68

    Principles for Derivation of Human Embryonic Stem Cells

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    Book Protocol

    Pages 69-80

    Derivation and Maintenance of Undifferentiated Human Embryonic Stem Cells

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    Book Protocol

    Pages 81-112

    Establishment of hESC Lines from the Inner Cell Mass of Blastocyst-Stage Embryos and Single Blastomeres of 4-Cell Stage Embryos

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    Book Protocol

    Pages 113-125

    Analysis of LINE-1 Expression in Human Pluripotent Cells

  9. No Access

    Book Protocol

    Pages 127-149

    Characterization and Gene Expression Profiling of Five Human Embryonic Stem Cell Lines Derived in Taiwan

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    Book Protocol

    Pages 151-161

    Derivation of Human Embryonic Stem Cell Lines from Poor Quality Embryos

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    Book Protocol

    Pages 163-178

    Derivation, Expansion, and Characterization of Human Embryonic Stem Cell Lines from Aneuploid Embryos

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    Book Protocol

    Pages 179-207

    Mutated Human Embryonic Stem Cells for the Study of Human Genetic Disorders

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    Book Protocol

    Pages 209-215

    Single-Cell Enzymatic Dissociation of hESC Lines OxF1–OxF4 and Culture in Feeder-Free Conditions

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    Book Protocol

    Pages 217-226

    Protocol for Expansion of Undifferentiated Human Embryonic and Pluripotent Stem Cells in Suspension

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    Book Protocol

    Pages 227-235

    Suspension Bioreactor Expansion of Undifferentiated Human Embryonic Stem Cells

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    Book Protocol

    Pages 237-246

    Derivation, Propagation, and Characterization of Neuroprogenitors from Pluripotent Stem Cells (hESCs and hiPSCs)

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    Book Protocol

    Pages 247-259

    Comparison of Neural Differentiation Potential of Human Pluripotent Stem Cell Lines Using a Quantitative Neural Differentiation Protocol

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    Book Protocol

    Pages 261-267

    Array-Comparative Genomic Hybridization Characterization of Human Pluripotent Stem Cells

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    Book Protocol

    Pages 269-280

    Chromatin Immunoprecipitation-Based Analysis of Gene Regulatory Networks Operative in Human Embryonic Stem Cells

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    Book Protocol

    Pages 281-295

    Analysis of the Methylome of Human Embryonic Stem Cells Employing Methylated DNA Immunoprecipitation Coupled to Next-Generation Sequencing

  21. No Access

    Book Protocol

    Pages 297-305

    Stable Isotope Labelling with Amino Acids in Cell Culture for Human Embryonic Stem Cell Proteomic Analysis

  22. Back Matter

    Pages 307-313