Lipases and Phospholipases

Methods and Protocols

Editors:

ISBN: 978-1-61779-599-2 (Print) 978-1-61779-600-5 (Online)

Table of contents (30 protocols)

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  1. Front Matter

    Pages i-xv

  2. OVERVIEW OF LIPASES, PHOSPHOLIPASES AND RELATED ENZYMES

    1. Front Matter

      Pages 1-1

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      Pages 3-30

      Lipases: An Overview

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      Pages 31-51

      Lipases or Esterases: Does It Really Matter? Toward a New Bio-Physico-Chemical Classification

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      Pages 53-61

      Study of New Feruloyl Esterases to Understand Lipase Evolution: The Case of Bacillus flexus

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      Pages 63-85

      Phospholipases: An Overview

  3. SCREENING AND SOURCES OF LIPASES, PHOSPHOLIPASES AND ITS INHIBITORS

    1. Front Matter

      Pages 87-87

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      Pages 89-100

      High-Throughput Screening Method for Lipases/Esterases

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      Pages 101-113

      Functional-Based Screening Methods for Lipases, Esterases, and Phospholipases in Metagenomic Libraries

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      Pages 115-122

      Plant Lipases: Partial Purification of Carica papaya Lipase

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      Pages 123-137

      Plant Phospholipases: An Overview

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      Pages 139-148

      Lipase and Phospholipase Inhibitors: Design and Applications

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      Pages 149-158

      High-Throughput Assay of Secreted Phospholipases A2 Inhibitors

  4. CLONING, PRODUCTION, PURIFICATION AND CHARACTERIZATION OF LIPASES AND PHOSPHOLIPASES

    1. Front Matter

      Pages 159-159

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      Pages 161-178

      Heterologous Expression Systems for Lipases: A Review

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      Pages 179-200

      Streptomyces Phospholipase D Cloning and Production

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      Pages 201-225

      Cloning of PLD2 from Baculovirus for Studies in Inflammatory Responses

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      Pages 227-237

      Lipases Production by Solid-State Fermentation: The Case of Rhizopus homothallicus in Perlite

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      Pages 239-266

      Lipases and Esterases from Extremophiles: Overview and Case Example of the Production and Purification of an Esterase from Thermus thermophilus HB27

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      Pages 267-282

      Purification, Biochemical and Kinetic Properties of Recombinant Staphylococcus aureus Lipase

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      Pages 283-297

      Phospholipase A2 Purification and Characterization: A Case Study

  5. OPTIMIZATION OF THE BIOCATALYST AND REACTION CONDITIONS

    1. Front Matter

      Pages 299-299

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      Pages 301-312

      The Yeast Yarrowia lipolytica as a Generic Tool for Molecular Evolution of Enzymes

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      Pages 313-327

      Molecular Modeling of Lipase Binding to a Substrate–Water Interface

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      Pages 329-341

      Different Strategies for Hyperactivation of Lipase Biocatalysts

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