Plant Metabolomics

Methods and Protocols

Editors:

ISBN: 978-1-61779-593-0 (Print) 978-1-61779-594-7 (Online)

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiii

  2. No Access

    Book Protocol

    Pages 1-10

    Practical Applications of Metabolomics in Plant Biology

  3. Material Preparation

    1. Front Matter

      Pages 11-11

    2. No Access

      Book Protocol

      Pages 13-30

      Aspects of Experimental Design for Plant Metabolomics Experiments and Guidelines for Growth of Plant Material

    3. No Access

      Book Protocol

      Pages 31-49

      Separating the Inseparable: The Metabolomic Analysis of Plant–Pathogen Interactions

    4. No Access

      Book Protocol

      Pages 51-63

      Precautions for Harvest, Sampling, Storage, and Transport of Crop Plant Metabolomics Samples

    5. No Access

      Book Protocol

      Pages 65-81

      Tissue Preparation Using Arabidopsis

  4. Chemical Analysis Approaches

    1. Front Matter

      Pages 83-83

    2. No Access

      Book Protocol

      Pages 85-99

      Solid Phase Micro-Extraction GC–MS Analysis of Natural Volatile Components in Melon and Rice

    3. No Access

      Book Protocol

      Pages 101-109

      Profiling Primary Metabolites of Tomato Fruit with Gas Chromatography/Mass Spectrometry

    4. No Access

      Book Protocol

      Pages 111-128

      High-Performance Liquid Chromatography–Mass Spectrometry Analysis of Plant Metabolites in Brassicaceae

    5. No Access

      Book Protocol

      Pages 129-144

      UPLC-MS-Based Metabolite Analysis in Tomato

    6. No Access

      Book Protocol

      Pages 145-156

      High Precision Measurement and Fragmentation Analysis for Metabolite Identification

    7. No Access

      Book Protocol

      Pages 157-176

      Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Plant Metabolite Profiling and Metabolite Identification

    8. No Access

      Book Protocol

      Pages 177-191

      Combined NMR and Flow Injection ESI-MS for Brassicaceae Metabolomics

    9. No Access

      Book Protocol

      Pages 193-211

      ICP-MS and LC-ICP-MS for Analysis of Trace Element Content and Speciation in Cereal Grains

    10. No Access

      Book Protocol

      Pages 213-226

      The Use of Genomics and Metabolomics Methods to Quantify Fungal Endosymbionts and Alkaloids in Grasses

  5. Data Analysis

    1. Front Matter

      Pages 227-227

    2. No Access

      Book Protocol

      Pages 229-253

      Data (Pre-)processing of Nominal and Accurate Mass LC-MS or GC-MS Data Using MetAlign

    3. No Access

      Book Protocol

      Pages 255-286

      TagFinder: Preprocessing Software for the Fingerprinting and the Profiling of Gas Chromatography–Mass Spectrometry Based Metabolome Analyses

    4. No Access

      Book Protocol

      Pages 287-316

      Chemical Identification Strategies Using Liquid Chromatography-Photodiode Array-Solid-Phase Extraction-Nuclear Magnetic Resonance/Mass Spectrometry

    5. No Access

      Book Protocol

      Pages 317-333

      A Strategy for Selecting Data Mining Techniques in Metabolomics

  6. Back Matter

    Pages 335-340