Difference Gel Electrophoresis (DIGE)

Methods and Protocols

Editors:

ISBN: 978-1-61779-572-5 (Print) 978-1-61779-573-2 (Online)

Table of contents (26 protocols)

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  1. Front Matter

    Pages i-xiii

  2. Fundamentals

    1. Front Matter

      Pages 1-1

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      Pages 3-8

      DIGE: Past and Future

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      Pages 9-19

      The Basics of 2D DIGE

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      Pages 21-30

      Multifluorescence 2D Gel Imaging and Image Analysis

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      Pages 31-45

      Assessing Signal-to-Noise in Quantitative Proteomics: Multivariate Statistical Analysis in DIGE Experiments

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      Pages 47-66

      Analysis of Proteins Using DIGE and MALDI Mass Spectrometry

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      Pages 67-85

      Synthesis and Validation of Cyanine-Based Dyes for DIGE

  3. Methods

    1. Front Matter

      Pages 87-87

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      Book Protocol

      Pages 89-112

      2D DIGE Saturation Labeling for Minute Sample Amounts

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      Pages 113-128

      Proteomic Analysis of Redox-Dependent Changes Using Cysteine-Labeling 2D DIGE

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      Pages 129-143

      Analysis of Protein Posttranslational Modifications Using DIGE-Based Proteomics

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      Pages 145-154

      Comparative Analyses of Protein Complexes by Blue Native DIGE

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      Pages 155-168

      2D DIGE Analysis of Protein Extracts from Muscle Tissue

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      Pages 169-180

      Combination of Highly Efficient Hexapeptide Ligand Library-Based Sample Preparation with 2D DIGE for the Analysis of the Hidden Human Serum/Plasma Proteome

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      Pages 181-194

      2D DIGE Analysis of Serum After Fractionation by ProteoMiner™ Beads

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      Pages 195-206

      Study Design in DIGE-Based Biomarker Discovery

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      Pages 207-220

      Comparative 2D DIGE Analysis of the Depleted Serum Proteome for Biomarker Discovery

  4. Applications in Clinical Proteomics

    1. Front Matter

      Pages 221-221

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      Book Protocol

      Pages 223-237

      Differential Gel-Based Proteomic Approach for Cancer Biomarker Discovery Using Human Plasma

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      Pages 239-252

      2D DIGE for the Analysis of RAMOS Cells Subproteomes

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      Pages 253-267

      Application of Saturation Labeling in Lung Cancer Proteomics

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      Pages 269-286

      Proteomic Profiling of the Epithelial-Mesenchymal Transition Using 2D DIGE

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      Pages 287-297

      Method for Protein Subfractionation of Cardiovascular Tissues Before DIGE Analysis

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