Gene Synthesis

Methods and Protocols

Editors:

ISBN: 978-1-61779-563-3 (Print) 978-1-61779-564-0 (Online)

Table of contents (21 protocols)

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  1. Front Matter

    Pages i-xi

  2. Assembly of oligonucleotides in synthons

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-10

      Building Block Synthesis Using the Polymerase Chain Assembly Method

    3. No Access

      Book Protocol

      Pages 11-21

      Oligonucleotide Assembly in Yeast to Produce Synthetic DNA Fragments

    4. No Access

      Book Protocol

      Pages 23-34

      TopDown Real-Time Gene Synthesis

    5. No Access

      Book Protocol

      Pages 35-47

      De Novo DNA Synthesis Using Single-Molecule PCR

  3. Synthon Assembly

    1. Front Matter

      Pages 49-49

    2. No Access

      Book Protocol

      Pages 51-59

      SLIC: A Method for Sequence- and Ligation-Independent Cloning

    3. No Access

      Book Protocol

      Pages 61-76

      Assembly of Standardized DNA Parts Using BioBrick Ends in E. coli

    4. No Access

      Book Protocol

      Pages 77-95

      Assembling DNA Fragments by USER Fusion

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      Book Protocol

      Pages 97-110

      Fusion PCR via Novel Overlap Sequences

    6. No Access

      Book Protocol

      Pages 111-120

      Using Recombineering to Generate Point Mutations: The Oligonucleotide-Based “Hit and Fix” Method

    7. No Access

      Book Protocol

      Pages 121-131

      Using Recombineering to Generate Point Mutations:galK-Based Positive–Negative Selection Method

    8. No Access

      Book Protocol

      Pages 133-150

      Assembling Large DNA Segments in Yeast

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      Book Protocol

      Pages 151-163

      Recursive Construction of Perfect DNA Molecules and Libraries from Imperfect Oligonucleotides

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      Pages 165-180

      Cloning Whole Bacterial Genomes in Yeast

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      Pages 181-193

      Production of Infectious Poliovirus from Synthetic Viral Genomes

  4. Software for gene synthesis

    1. Front Matter

      Pages 195-195

    2. No Access

      Book Protocol

      Pages 197-213

      In Silico Design of Functional DNA Constructs

    3. No Access

      Book Protocol

      Pages 215-223

      Using DNAWorks in Designing Oligonucleotides for PCR-Based Gene Synthesis

    4. No Access

      Book Protocol

      Pages 225-234

      De Novo Gene Synthesis Design Using TmPrime Software

    5. No Access

      Book Protocol

      Pages 235-247

      Design-A-Gene with GeneDesign

  5. Education and Security

    1. Front Matter

      Pages 249-249

    2. No Access

      Book Protocol

      Pages 251-272

      Leading a Successful iGEM Team

    3. No Access

      Book Protocol

      Pages 273-283

      The Build-a-Genome Course

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