Transgenic Plants

Methods and Protocols

Editors:

ISBN: 978-1-61779-557-2 (Print) 978-1-61779-558-9 (Online)

Table of contents (37 protocols)

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  1. Front Matter

    Pages i-xv

  2. SELECTION AND DETECTION METHODS

    1. Front Matter

      Pages 1-1

    2. No Access

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      Pages 3-10

      Employment of Cytokinin Vectors for Marker-Free and Backbone-Free Transformation

    3. No Access

      Book Protocol

      Pages 11-23

      Organophosphorus Hydrolase: A Multifaceted Plant Genetic Marker Which Is Selectable, Scorable, and Quantifiable in Whole Seed

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      Pages 25-32

      Use of Northern Blotting for Specific Detection of Small RNA Molecules in Transgenic Plants

  3. TRANSFORMATION TECHNOLOGY – LOWER PLANTS

    1. Front Matter

      Pages 33-33

    2. No Access

      Book Protocol

      Pages 35-47

      Genetic Transformation of the Model Green Alga Chlamydomonas reinhardtii

  4. TRANSFORMATION TECHNOLOGY – RICE

    1. Front Matter

      Pages 49-49

    2. No Access

      Book Protocol

      Pages 51-57

      A High-Efficiency Agrobacterium-Mediated Transformation System of Rice (Oryza sativa L.)

    3. No Access

      Book Protocol

      Pages 59-66

      Selection of Transgenic Rice Plants Using a Herbicide Tolerant Form of the Acetolactate Synthase Gene

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      Book Protocol

      Pages 67-74

      Visual Selection in Rice: A Strategy for the Efficient Identification of Transgenic Calli Accumulating Transgene Products

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      Book Protocol

      Pages 75-90

      Characterization of Rice Genes Using a Heterologous Full-Length cDNA Expression System

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      Pages 91-106

      Bioactive Bead-Mediated Transformation of Plants with Large DNA Fragments

  5. TRANSFORMATION TECHNOLOGY – CEREALS AND OTHER MONOCOTS

    1. Front Matter

      Pages 107-107

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      Book Protocol

      Pages 109-122

      Agrobacterium-Mediated Transformation of Sorghum bicolor Using Immature Embryos

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      Book Protocol

      Pages 123-135

      Split-Transgene Expression in Wheat

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      Pages 137-149

      Agrobacterium-Mediated Transformation of Brachypodium distachyon

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      Book Protocol

      Pages 151-161

      Transformation of Barley (Hordeum vulgare L.) by Agrobacterium tumefaciens Infection of In Vitro Cultured Ovules

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      Book Protocol

      Pages 163-175

      Biolistic-Mediated Production of Transgenic Oil Palm

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      Pages 177-188

      Transformation of Oil Palm Using Agrobacterium tumefaciens

  6. TRANSFORMATION TECHNOLOGY – DICOTS

    1. Front Matter

      Pages 189-189

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      Book Protocol

      Pages 191-199

      Highly Efficient Transformation Protocol for Plum (Prunus domestica L.)

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      Book Protocol

      Pages 201-213

      Co-transformation of Grapevine Somatic Embryos to Produce Transgenic Plants Free of Marker Genes

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      Book Protocol

      Pages 215-225

      Initiation and Transformation of Grapevine Embryogenic Cultures

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      Book Protocol

      Pages 227-235

      Development of Highly Efficient Genetic Transformation Protocols for Table Grape Sugraone and Crimson Seedless

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      Book Protocol

      Pages 237-243

      Cotton Pistil Drip Transformation Method

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