Host-Fungus Interactions

Methods and Protocols

Editors:

ISBN: 978-1-61779-538-1 (Print) 978-1-61779-539-8 (Online)

Table of contents (42 protocols)

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  1. Front Matter

    Pages i-xvi

  2. Gene Disruption

    1. Front Matter

      Pages 1-1

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      Pages 3-17

      Gene Deletion in Candida albicans Wild-Type Strains Using the SAT1-Flipping Strategy

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      Pages 19-39

      Mini-blaster-Mediated Targeted Gene Disruption and Marker Complementation in Candida albicans

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      Pages 41-49

      Rapid Detection of Aneuploidy Following the Generation of Mutants in Candida albicans

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      Pages 51-66

      Agrobacterium-Mediated Insertional Mutagenesis in Histoplasma capsulatum

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      Pages 67-84

      Targeted Gene Disruption in Cryptococcus neoformans Using Double-Joint PCR with Split Dominant Selectable Markers

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      Pages 85-98

      Multiple Gene Deletion in Cryptococcus neoformans Using the Cre–lox System

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      Pages 99-118

      Gene Disruption in Aspergillus fumigatus Using a PCR-Based Strategy and In Vivo Recombination in Yeast

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      Pages 119-130

      Targeted Gene Deletion in Aspergillus fumigatus Using the Hygromycin-Resistance Split-Marker Approach

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      Pages 131-147

      Gene Disruption in Coccidioides Using Hygromycin or Phleomycin Resistance Markers

  3. Modulation of Gene Expression: RNAi Gene Knockdown

    1. Front Matter

      Pages 149-149

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      Pages 151-164

      RNAi-Based Gene Silencing Using a GFP Sentinel System in Histoplasma capsulatum

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      Pages 165-186

      RNA Interference in Cryptococcus neoformans

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      Pages 187-198

      Gene Knockdown in Paracoccidioides brasiliensis Using Antisense RNA

  4. Modulation of Gene Expression: Regulatable Promoters

    1. Front Matter

      Pages 199-199

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      Pages 201-210

      Tetracycline-Inducible Gene Expression in Candida albicans

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      Pages 211-226

      Galactose-Inducible Promoters in Cryptococcus neoformans var. grubii

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      Pages 227-244

      Modular Gene Over-expression Strategies for Candida albicans

  5. Responses to Infection In Vivo

    1. Front Matter

      Pages 245-245

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      Pages 247-260

      Interactions Between Macrophages and Cell Wall Oligosaccharides of Candida albicans

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      Pages 261-275

      Murine Bone Marrow-Derived Dendritic Cells and T-Cell Activation by Candida albicans

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      Pages 277-287

      Phagocytosis and Intracellular Killing of Candida albicans by Murine Polymorphonuclear Neutrophils

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      Pages 289-302

      Human Oral Keratinocytes: A Model System to Analyze Host–Pathogen Interactions

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      Pages 303-317

      Simple Assays for Measuring Innate Interactions with Fungi

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