Leucocytes

Methods and Protocols

Editors:

ISBN: 978-1-61779-526-8 (Print) 978-1-61779-527-5 (Online)

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi

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    Pages 1-13

    ENU-Based Phenotype-Driven Screening

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    Pages 15-30

    Detection and Quantification of Cytokines and Other Biomarkers

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    Pages 31-43

    Flow Cytometry Analysis of Cell Cycling and Proliferation in Mouse Hematopoietic Stem and Progenitor Cells

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    Pages 45-63

    Flow Cytometry Measurement of Bone Marrow Perfusion in the Mouse and Sorting of Progenitors and Stems Cells According to Position Relative to Blood Flow In Vivo

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    Pages 65-86

    Analyzing Cell Death Events in Cultured Leukocytes

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    Pages 87-99

    Towards a Four-Dimensional View of Neutrophils

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    Pages 101-113

    Isolation of Human and Mouse Neutrophils Ex Vivo and In Vitro

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    Pages 115-124

    Measurement of Oxidative Burst in Neutrophils

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    Pages 125-138

    Measurement of Neutrophil Elastase, Proteinase 3, and Cathepsin G Activities using Intramolecularly Quenched Fluorogenic Substrates

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    Pages 139-156

    The Macrophage

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    Pages 157-176

    Generation and Characterization of MacGreen Mice, the Cfs1r-EGFP Transgenic Mice

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    Pages 177-181

    Generation of Mouse Bone Marrow-Derived Macrophages

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    Pages 183-187

    Isolation and Differentiation of Monocytes–Macrophages from Human Blood

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    Pages 189-197

    In Vitro Measurement of Phagocytosis and Killing of Cryptococcus neoformans by Macrophages

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    Pages 199-222

    Measuring the Inflammasome

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    Pages 223-235

    Arginine and Macrophage Activation

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    Pages 237-250

    Immunodetection of Granzyme B Tissue Distribution and Cellular Localisation

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    Pages 251-260

    Detection of Human and Mouse Granzyme B Activity in Cell Extracts

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    Pages 261-275

    T Cell Transfer Model of Colitis: A Great Tool to Assess the Contribution of T Cells in Chronic Intestinal Inflammation

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    Pages 277-293

    Measurement of Nitrite in Urine by Gas Chromatography-Mass Spectrometry

  22. Back Matter

    Pages 295-296