Ancient DNA

Methods and Protocols

Editors:

ISBN: 978-1-61779-515-2 (Print) 978-1-61779-516-9 (Online)

Table of contents (24 protocols)

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  1. Front Matter

    Pages i-xii

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    Pages 1-11

    Setting Up an Ancient DNA Laboratory

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    Pages 13-19

    A Phenol–Chloroform Protocol for Extracting DNA from Ancient Samples

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    Pages 21-28

    DNA Extraction of Ancient Animal Hard Tissue Samples via Adsorption to Silica Particles

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    Pages 29-35

    Case Study: Recovery of Ancient Nuclear DNA from Toe Pads of the Extinct Passenger Pigeon

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    Pages 37-42

    Extraction of DNA from Paleofeces

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    Pages 43-49

    DNA Extraction from Keratin and Chitin

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    Pages 51-56

    Case Study: Ancient Sloth DNA Recovered from Hairs Preserved in Paleofeces

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    Pages 57-63

    Ancient DNA Extraction from Soils and Sediments

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    Pages 65-70

    DNA Extraction from Fossil Eggshell

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    Pages 71-79

    Ancient DNA Extraction from Plants

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    Pages 81-85

    DNA Extraction from Formalin-Fixed Material

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    Pages 87-92

    Case Study: Ancient DNA Recovered from Pleistocene-Age Remains of a Florida Armadillo

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    Pages 93-100

    Nondestructive DNA Extraction from Museum Specimens

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    Pages 101-110

    Case Study: Using a Nondestructive DNA Extraction Method to Generate mtDNA Sequences from Historical Chimpanzee Specimens

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    Pages 111-119

    PCR Amplification, Cloning, and Sequencing of Ancient DNA

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    Pages 121-132

    Quantitative Real-Time PCR in aDNA Research

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    Pages 133-141

    Multiplex PCR Amplification of Ancient DNA

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    Pages 143-154

    Preparation of Next-Generation Sequencing Libraries from Damaged DNA

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    Pages 155-170

    Generating Barcoded Libraries for Multiplex High-Throughput Sequencing

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    Pages 171-176

    Case Study: Targeted high-Throughput Sequencing of Mitochondrial Genomes from Extinct Cave Bears via Direct Multiplex PCR Sequencing (DMPS)

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