Mucins

Methods and Protocols

Editors:

ISBN: 978-1-61779-512-1 (Print) 978-1-61779-513-8 (Online)

Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi

  2. No Access

    Book Protocol

    Pages 1-26

    Mucin Methods: Genes Encoding Mucins and Their Genetic Variation with a Focus on Gel-Forming Mucins

  3. No Access

    Book Protocol

    Pages 27-47

    Gel-Forming and Cell-Associated Mucins: Preparation for Structural and Functional Studies

  4. No Access

    Book Protocol

    Pages 49-66

    Detecting, Visualising, and Quantifying Mucins

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    Book Protocol

    Pages 67-79

    Mass Spectrometric Analysis of Mucin Core Proteins

  6. No Access

    Book Protocol

    Pages 81-108

    O-Glycoprotein Biosynthesis: Site Localization by Edman Degradation and Site Prediction Based on Random Peptide Substrates

  7. No Access

    Book Protocol

    Pages 109-121

    Analysis of Assembly of Secreted Mucins

  8. No Access

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    Pages 123-140

    MUC1 Membrane Trafficking: Protocols for Assessing Biosynthetic Delivery, Endocytosis, Recycling, and Release Through Exosomes

  9. No Access

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    Pages 141-163

    Glycomic Work-Flow for Analysis of Mucin O-Linked Oligosaccharides

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    Pages 165-177

    O-Glycomics: Profi ling and Structural Analysis of Mucin-type O-linked Glycans

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    Pages 179-189

    O-Glycoproteomics: Site-Specifi c O-Glycoprotein Analysis by CID/ETD Electrospray Ionization Tandem Mass Spectrometry and Top-Down Glycoprotein Sequencing by In-Source Decay MALDI Mass Spectrometry

  12. No Access

    Book Protocol

    Pages 191-215

    Analysing Mucin Degradation

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    Book Protocol

    Pages 217-227

    Assessment of Mucus Thickness and Production In Situ

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    Pages 229-235

    Preservation of Mucus in Histological Sections, Immunostaining of Mucins in Fixed Tissue, and Localization of Bacteria with FISH

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    Book Protocol

    Pages 237-243

    Ex Vivo Measurements of Mucus Secretion by Colon Explants

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    Book Protocol

    Pages 245-258

    Establishment of Respiratory Air–Liquid Interface Cultures and Their Use in Studying Mucin Production, Secretion, and Function

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    Book Protocol

    Pages 259-277

    Studying Mucin Secretion from Human Bronchial Epithelial Cell Primary Cultures

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    Book Protocol

    Pages 279-295

    Assessment of Intracellular Mucin Content In Vivo

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    Book Protocol

    Pages 297-312

    Techniques for Assessment of Interactions of Mucins with Microbes and Parasites In Vitro and In Vivo

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    Book Protocol

    Pages 313-325

    Assessing Mucin Expression and Function in Human Ocular Surface Epithelia In Vivo and In Vitro

  21. Back Matter

    Pages 327-329