Planar Cell Polarity

Methods and Protocols

Editors:

ISBN: 978-1-61779-509-1 (Print) 978-1-61779-510-7 (Online)

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi

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    Pages 1-17

    Live Imaging of Drosophila Embryos: Quantifying Protein Numbers and Dynamics at Subcellular Locations

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    Pages 19-25

    Analyzing Frizzled Signaling Using Fixed and Live Imaging of the Asymmetric Cell Division of the Drosophila Sensory Organ Precursor Cell

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    Pages 27-41

    Protein–Protein Interaction Techniques: Dissect PCP Signaling in Xenopus

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    Pages 43-52

    Cuticle Refraction Microscopy: A Rapid and Simple Method for Imaging Drosophila Wing Topography, an Alternative Readout of Wing Planar Cell Polarity

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    Pages 53-68

    Analysis of Cell Shape and Polarity During Zebrafish Gastrulation

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    Pages 69-78

    Analyzing Planar Cell Polarity During Zebrafish Gastrulation

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    Pages 79-89

    Wnt/Planar Cell Polarity Signaling in the Regulation of Convergent Extension Movements During Xenopus Gastrulation

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    Pages 91-104

    Using 32-Cell Stage Xenopus Embryos to Probe PCP Signaling

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    Pages 105-117

    Gene Loss-of-Function and Live Imaging in Chick Embryos

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    Pages 119-131

    Activation and Function of Small GTPases Rho, Rac, and Cdc42 During Gastrulation

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    Pages 133-146

    Convergent Extension Analysis in Mouse Whole Embryo Culture

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    Pages 147-156

    Analysis of PCP Defects in Mammalian Eye Lens

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    Pages 157-171

    Examining Planar Cell Polarity in the Mammalian Cochlea

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    Pages 173-185

    Role of Prickle1 and Prickle2 in Neurite Outgrowth in Murine Neuroblastoma Cells

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    Pages 187-200

    The Planar Cell Polarity Pathway and Parietal Endoderm Cell Migration

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    Pages 201-214

    Analysis of Wnt/Planar Cell Polarity Pathway in Cultured Cells

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    Pages 215-227

    Regulation of Focal Adhesion Dynamics by Wnt5a Signaling

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    Pages 229-237

    The Embryonic Mouse Gut Tube as a Model for Analysis of Epithelial Polarity

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    Pages 239-248

    Assessing PCP in the Cochlea of Mammalian Ciliopathy Models

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    Pages 249-255

    Morphometric Analysis of Centrosome Position in Tissues

  22. Back Matter

    Pages 257-262