Plant Fungal Pathogens

Methods and Protocols

Editors:

ISBN: 978-1-61779-500-8 (Print) 978-1-61779-501-5 (Online)

Table of contents (41 protocols)

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  1. Front Matter

    Pages i-xvi

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    Pages 1-16

    Fungal ABC Transporter Deletion and Localization Analysis

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    Pages 17-45

    Targeted Gene Replacement in Fungal Pathogens via Agrobacterium tumefaciens- Mediated Transformation

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    Pages 47-59

    Activity-Based Protein Profiling of Infected Plants

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    Pages 61-74

    The Use of Agroinfiltration for Transient Expression of Plant Resistance and Fungal Effector Proteins in Nicotiana benthamiana Leaves

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    Pages 75-96

    Proteomic Techniques for Plant–Fungal Interactions

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    Pages 97-105

    Identification of HR-Inducing cDNAs from Plant Pathogens via a Gateway®-Compatible Binary Potato Virus X-Expression Vector

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    Pages 107-119

    Freeze-Fracture of Infected Plant Leaves in Ethanol for Scanning Electron Microscopic Study of Fungal Pathogens

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    Pages 121-132

    Real-Time PCR for the Quantification of Fungi In Planta

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    Pages 133-150

    Using Non-homologous End-Joining-Deficient Strains for Functional Gene Analyses in Filamentous Fungi

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    Pages 151-164

    Atomic Force Microscopy: A Tool for Studying Biophysical Surface Properties Underpinning Fungal Interactions with Plants and Substrates

  12. No Access

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    Pages 165-189

    Use of the Yeast Two-Hybrid System to Identify Targets of Fungal Effectors

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    Pages 191-207

    Characterization of Plant-Fungal Interactions Involving Necrotrophic Effector-Producing Plant Pathogens

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    Pages 209-217

    Heterologous Production of Fungal Effectors in Pichia pastoris

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    Pages 219-236

    The Application of Laser Microdissection to Profiling Fungal Pathogen Gene Expression in planta

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    Pages 237-254

    Metabolomics Protocols for Filamentous Fungi

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    Pages 255-269

    Targeted Gene Replacement in Fungi Using a Split-Marker Approach

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    Pages 271-294

    Massively Parallel Sequencing Technology in Pathogenic Microbes

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    Pages 295-309

    Confocal Microscopy in Plant–Pathogen Interactions

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    Pages 311-331

    The Use of Open Source Bioinformatics Tools to Dissect Transcriptomic Data

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    Pages 333-363

    Population Biology of Fungal Plant Pathogens

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