Proteoglycans

Methods and Protocols

Editors:

ISBN: 978-1-61779-497-1 (Print) 978-1-61779-498-8 (Online)

Table of contents (22 protocols)

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  1. Front Matter

    Pages i-xiv

  2. Proteoglycans

    1. Front Matter

      Pages 1-1

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      Pages 3-21

      Proteoglycans: Gene Cloning

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      Pages 23-34

      Proteoglycan: Site Mapping and Site-Directed Mutagenesis

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      Pages 35-52

      Mapping of the Wnt/β-Catenin/TCF Response Elements in the Human Versican Promoter

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      Pages 53-61

      Gene Silencing in Mouse Embryonic Stem Cells

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      Pages 63-85

      A Novel Strategy for a Splice-Variant Selective Gene Ablation: The Example of the Versican V0/V2 Knockout

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      Pages 87-95

      Detection of Neurocan in Cerebrospinal Fluid

  3. Glycosaminoglycans Analysis

    1. Front Matter

      Pages 97-97

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      Pages 99-115

      Glycosaminoglycan Chain Analysis and Characterization (Glycosylation/Epimerization)

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      Pages 117-130

      Characterization of Glycosaminoglycans by Tandem Vibrational Microspectroscopy and Multivariate Data Analysis

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      Pages 131-144

      Glycosaminoglycans: Oligosaccharide Analysis by Liquid Chromatography, Capillary Electrophoresis, and Specific Labeling

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      Pages 145-159

      Brain Chondroitin/Dermatan Sulfate, from Cerebral Tissue to Fine Structure: Extraction, Preparation, and Fully Automated Chip-Electrospray Mass Spectrometric Analysis

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      Pages 161-169

      Use of Neutrons Reveals the Dynamics of Cell Surface Glycosaminoglycans

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      Pages 171-182

      Following Protein–Glycosaminoglycan Polysaccharide Interactions with Differential Scanning Fluorimetry

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      Pages 183-198

      In Vivo Scintigraphic Imaging of Proteoglycans

  4. PGs: Multifunctional Cell Regulators

    1. Front Matter

      Pages 199-199

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      Pages 201-217

      Serglycin: The Master of the Mast Cell

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      Pages 219-237

      Analysis of Aggrecan Catabolism by Immunoblotting and Immunohistochemistry

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      Pages 239-255

      Heparan Sulfate Proteoglycans as Multifunctional Cell Regulators: Cell Surface Receptors

  5. Proteoglycans Involvement in Pathophysiology

    1. Front Matter

      Pages 257-257

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      Pages 259-284

      Models for Studies of Proteoglycans in Kidney Pathophysiology

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      Pages 285-290

      Lumican Promotes Corneal Epithelial Wound Healing

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      Pages 291-305

      Shedding of Cell Membrane-Bound Proteoglycans

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      Pages 307-322

      Modulatory Effects of Proteoglycans on Proteinase Activities

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