Microbial Metabolic Engineering

Methods and Protocols

Editors:

ISBN: 978-1-61779-482-7 (Print) 978-1-61779-483-4 (Online)

Table of contents (19 protocols)

  1. Front Matter

    Pages i-xii

  2. No Access

    Book Protocol

    Pages 1-16

    Screening for Cellulases with Industrial Value and Their Use in Biomass Conversion

  3. No Access

    Book Protocol

    Pages 17-31

    Reversal of NAD(P)H Cofactor Dependence by Protein Engineering

  4. No Access

    Book Protocol

    Pages 33-48

    Quantifying Plasmid Copy Number to Investigate Plasmid Dosage Effects Associated with Directed Protein Evolution

  5. No Access

    Book Protocol

    Pages 49-62

    High Isoprenoid Flux Escherichia coli as a Host for Carotenoids Production

  6. No Access

    Book Protocol

    Pages 63-73

    Mutagenic Inverted Repeats Assisted Genome Engineering (MIRAGE) in Saccharomyces cerevisiae: Deletion of gal7

  7. No Access

    Book Protocol

    Pages 75-86

    Creation of New Metabolic Pathways or Improvement of Existing Metabolic Enzymes by In Vivo Evolution in Escherichia coli

  8. No Access

    Book Protocol

    Pages 87-92

    Bioluminescent Reporter Genes for Promoter Discovery

  9. No Access

    Book Protocol

    Pages 93-109

    Recombination-Based DNA Assembly and Mutagenesis Methods for Metabolic Engineering

  10. No Access

    Book Protocol

    Pages 111-136

    Ethanol-Tolerant Gene Identification in Clostridium thermocellum Using Pyro-Resequencing for Metabolic Engineering

  11. No Access

    Book Protocol

    Pages 137-151

    Use of Proteomic Tools in Microbial Engineering for Biofuel Production

  12. No Access

    Book Protocol

    Pages 153-175

    Metabolic Engineering of Antibiotic-Producing Actinomycetes Using In Vitro Transposon Mutagenesis

  13. No Access

    Book Protocol

    Pages 177-196

    Use FACS Sorting in Metabolic Engineering of Escherichia coli for Increased Peptide Production

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    Book Protocol

    Pages 197-216

    Using Flux Balance Analysis to Guide Microbial Metabolic Engineering

  15. No Access

    Book Protocol

    Pages 217-231

    Using an Advanced Microfermentor System for Strain Screening and Fermentation Optimization

  16. No Access

    Book Protocol

    Pages 233-244

    Rapid Strain Evaluation Using Dynamic DO-Stat Fed-Batch Fermentation Under Scale-Down Conditions

  17. No Access

    Book Protocol

    Pages 245-259

    Preparation and Evaluation of Lignocellulosic Biomass Hydrolysates for Growth by Ethanologenic Yeasts

  18. No Access

    Book Protocol

    Pages 261-281

    Engineering Whole-Cell Biosensors with No Antibiotic Markers for Monitoring Aromatic Compounds in the Environment

  19. No Access

    Book Protocol

    Pages 283-303

    Metabolic Engineering for Acetate Control in Large Scale Fermentation

  20. No Access

    Book Protocol

    Pages 305-315

    Minimization and Prevention of Phage Infections in Bioprocesses

  21. Back Matter

    Pages 317-319