Protein NMR Techniques

Editors:

ISBN: 978-1-61779-479-7 (Print) 978-1-61779-480-3 (Online)

Table of contents (24 protocols)

previous Page of 2
  1. Front Matter

    Pages i-xiv

  2. No Access

    Book Protocol

    Pages 1-18

    A Novel Bacterial Expression Method with Optimized Parameters for Very High Yield Production of Triple-Labeled Proteins

  3. No Access

    Book Protocol

    Pages 19-36

    Isotopic Labeling of Heterologous Proteins in the Yeast Pichia pastoris and Kluyveromyces lactis

  4. No Access

    Book Protocol

    Pages 37-54

    Isotope Labeling in Insect Cells

  5. No Access

    Book Protocol

    Pages 55-69

    Isotope Labeling in Mammalian Cells

  6. No Access

    Book Protocol

    Pages 71-84

    Cell-Free Protein Production for NMR Studies

  7. No Access

    Book Protocol

    Pages 85-109

    Cell-Free Membrane Protein Expression for Solid-State NMR

  8. No Access

    Book Protocol

    Pages 111-131

    Expression and Purification of Src-family Kinases for Solution NMR Studies

  9. No Access

    Book Protocol

    Pages 133-140

    NMR Studies of Large Protein Systems

  10. No Access

    Book Protocol

    Pages 141-163

    Protein Dynamics by 15N Nuclear Magnetic Relaxation

  11. No Access

    Book Protocol

    Pages 165-179

    Bacterial Production and Solution NMR Studies of a Viral Membrane Ion Channel

  12. No Access

    Book Protocol

    Pages 181-195

    Preparation of the Modular Multi-Domain Protein RPA for Study by NMR Spectroscopy

  13. No Access

    Book Protocol

    Pages 197-218

    NMR Studies of Protein–RNA Interactions

  14. No Access

    Book Protocol

    Pages 219-232

    Preparation and Optimization of Protein–DNA Complexes Suitable for Detailed NMR Studies

  15. No Access

    Book Protocol

    Pages 233-259

    NMR Studies of Protein–Ligand Interactions

  16. No Access

    Book Protocol

    Pages 261-277

    In-Cell NMR Spectroscopy in Escherichia coli

  17. No Access

    Book Protocol

    Pages 279-301

    Deuterated Peptides and Proteins: Structure and Dynamics Studies by MAS Solid-State NMR

  18. No Access

    Book Protocol

    Pages 303-331

    Solid-State NMR Spectroscopy of Protein Complexes

  19. No Access

    Book Protocol

    Pages 333-357

    Synthesis, Purification, and Characterization of Single Helix Membrane Peptides and Proteins for NMR Spectroscopy

  20. No Access

    Book Protocol

    Pages 359-368

    Assignment of Backbone Resonances in a Eukaryotic Protein Kinase – ERK2 as a Representative Example

  21. No Access

    Book Protocol

    Pages 369-405

    Electrostatics of Hydrogen Exchange for Analyzing Protein Flexibility

previous Page of 2