Astrocytes

Methods and Protocols

Editors:

ISBN: 978-1-61779-451-3 (Print) 978-1-61779-452-0 (Online)

Table of contents (37 protocols)

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  1. Front Matter

    Pages i-xiv

  2. Overview of Astrocyte Biology

    1. Front Matter

      Pages 1-1

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      Pages 3-7

      Astrocytes: Multitalented Stars of the Central Nervous System

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      Pages 9-22

      Neurogenic Astrocytes and Their Glycoconjugates: Not Just “Glue” Anymore

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      Pages 23-45

      Heterogeneity of Astrocytic Form and Function

  3. Isolation and Culture of Astrocytes

    1. Front Matter

      Pages 47-47

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      Pages 49-59

      Preparation of Mixed Glial Cultures from Postnatal Rat Brain

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      Pages 61-79

      Generation of Primary Astrocyte Cultures Devoid of Contaminating Microglia

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      Pages 81-91

      Derivation of Microglia-Free Astrocyte Cultures from Neural Stem Cells

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      Pages 93-104

      Isolation and Culture of Spinal Cord Astrocytes

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      Pages 105-115

      Isolation and Culture of Astrocytes from the Retina and Optic Nerve

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      Pages 117-126

      Primary Cultures of Astrocytes from Fetal Bovine Brain

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      Pages 127-135

      Isolation and Cultivation of Porcine Astrocytes

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      Pages 137-151

      Isolation and Culture of Human Astrocytes

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      Pages 153-174

      Cell Culturing of Caenorhabditis elegans Glial Cells for the Assessment of Cytosolic Ca2+ Dynamics

  4. Analysis of Astrocyte Functions In Vitro

    1. Front Matter

      Pages 175-175

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      Pages 177-188

      Astrocyte Polarization and Wound Healing in Culture: Studying Cell Adhesion Molecules

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      Pages 189-219

      An In Vitro Trauma Model to Study Rodent and Human Astrocyte Reactivity

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      Pages 221-233

      Use of Gel Zymography to Examine Matrix Metalloproteinase (Gelatinase) Expression in Brain Tissue or in Primary Glial Cultures

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      Pages 235-249

      ELISA Methodology to Quantify Astrocyte Production of Cytokines/Chemokines In Vitro

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      Pages 251-263

      Induction of Nitric Oxide Synthase-2 Expression and Measurement of Nitric Oxide Production in Enriched Primary Cortical Astrocyte Cultures

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      Pages 265-281

      Examining Potassium Channel Function in Astrocytes

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      Pages 283-303

      Connexin-Based Channels in Astrocytes: How to Study Their Properties

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      Pages 305-323

      Isotope-Based Quantitation of Uptake, Release, and Metabolism of Glutamate and Glucose in Cultured Astrocytes

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