Rho GTPases

Methods and Protocols

Editors:

ISBN: 978-1-61779-441-4 (Print) 978-1-61779-442-1 (Online)

Table of contents (26 protocols)

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  1. Front Matter

    Pages i-xiv

  2. History and Phylogenesis

    1. Front Matter

      Pages 1-1

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      Pages 3-12

      Historical Overview of Rho GTPases

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      Pages 13-34

      Rho GTPases: Deciphering the Evolutionary History of a Complex Protein Family

  3. Biochemistry of Rho GTPases

    1. Front Matter

      Pages 35-35

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      Pages 37-58

      Biochemical Assays to Characterize Rho GTPases

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      Pages 59-75

      How to Analyze Bacterial Toxins Targeting Rho GTPases

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      Pages 77-86

      Assessing Ubiquitylation of Rho GTPases in Mammalian Cells

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      Pages 87-95

      Posttranslational Lipid Modification of Rho Family Small GTPases

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      Pages 97-105

      Analysis of the Role of RhoGDI1 and Isoprenylation in the Degradation of RhoGTPases

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      Pages 107-119

      A Quantitative Fluorometric Approach for Measuring the Interaction of RhoGDI with Membranes and Rho GTPases

  4. Functional assays

    1. Front Matter

      Pages 121-121

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      Pages 123-142

      Rho GTPases and Cancer Cell Transendothelial Migration

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      Pages 143-155

      Quantitative and Robust Assay to Measure Cell–Cell Contact Assembly and Maintenance

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      Pages 157-166

      Rho GTPase Knockout Induction in Primary Keratinocytes from Adult Mice

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      Pages 167-179

      Rho GTPase Techniques in Osteoclastogenesis

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      Pages 181-194

      Assessment of Rho GTPase Signaling During Neurite Outgrowth

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      Pages 195-212

      Assessment of the Role for Rho Family GTPases in NADPH Oxidase Activation

  5. Imaging and high throughput methods

    1. Front Matter

      Pages 213-213

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      Pages 215-234

      Multiplex Imaging of Rho Family GTPase Activities in Living Cells

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      Pages 235-251

      FRET-Based Imaging of Rac and Cdc42 Activation During Fc-Receptor-Mediated Phagocytosis in Macrophages

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      Pages 253-270

      High-Throughput Flow Cytometry Bead-Based Multiplex Assay for Identification of Rho GTPase Inhibitors

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      Pages 271-282

      Functional Analysis of Rho GTPase Activation and Inhibition in a Bead-Based Miniaturized Format

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      Pages 283-303

      Use of Phage Display for the Identification of Molecular Sensors Specific for Activated Rho

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      Pages 305-317

      Identification of New Interacting Partners for Atypical Rho GTPases: A SILAC-Based Approach

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