Germline Development

Methods and Protocols

Editors:

ISBN: 978-1-61779-435-3 (Print) 978-1-61779-436-0 (Online)

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi

  2. Male Germline

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-16

      Isolation of Fetal Gonads from Embryos of Timed-Pregnant Mice for Morphological and Molecular Studies

    3. No Access

      Book Protocol

      Pages 17-29

      Neonatal Testicular Gonocytes Isolation and Processing for Immunocytochemical Analysis

    4. No Access

      Book Protocol

      Pages 31-44

      Isolation of Undifferentiated and Early Differentiating Type A Spermatogonia from Pou5f1-GFP Reporter Mice

    5. No Access

      Book Protocol

      Pages 45-57

      Isolation of Human Male Germ-Line Stem Cells Using Enzymatic Digestion and Magnetic-Activated Cell Sorting

    6. No Access

      Book Protocol

      Pages 59-66

      Isolation and Purification of Murine Male Germ Cells

    7. No Access

      Book Protocol

      Pages 67-74

      Preparation of Enriched Mouse Syncitia-Free Pachytene Spermatocyte Cell Suspensions

    8. No Access

      Book Protocol

      Pages 75-92

      Revealing the Transcriptome Landscape of Mouse Spermatogonial Cells by Tiling Microarray

    9. No Access

      Book Protocol

      Pages 93-104

      Biochemical Characterization of a Testis-Predominant Isoform of N-Alpha Acetyltransferase

    10. No Access

      Book Protocol

      Pages 105-114

      Identification of Novel Long Noncoding RNA Transcripts in Male Germ Cells

    11. No Access

      Book Protocol

      Pages 115-126

      Methylation Profiling Using Methylated DNA Immunoprecipitation and Tiling Array Hybridization

    12. No Access

      Book Protocol

      Pages 127-147

      Spermatogenesis in Cryptorchidism

  3. Female Germline

    1. Front Matter

      Pages 149-149

    2. No Access

      Book Protocol

      Pages 151-171

      In Vitro Culture of Fetal Ovaries: A Model to Study Factors Regulating Early Follicular Development

    3. No Access

      Book Protocol

      Pages 173-181

      Microspread Ovarian Cell Preparations for the Analysis of Meiotic Prophase Progression in Oocytes with Improved Recovery by Cytospin Centrifugation

    4. No Access

      Book Protocol

      Pages 183-198

      In Vitro Maturation (IVM) of Porcine Oocytes

    5. No Access

      Book Protocol

      Pages 199-210

      Experimental Approaches to the Study of Human Primordial Germ Cells

    6. No Access

      Book Protocol

      Pages 211-221

      Investigating the Origins of Somatic Cell Populations in the Perinatal Mouse Ovaries Using Genetic Lineage Tracing and Immunohistochemistry

    7. No Access

      Book Protocol

      Pages 223-235

      DNA Methylation Analysis of Germ Cells by Using Bisulfite-Based Sequencing Methods

    8. No Access

      Book Protocol

      Pages 237-251

      Gene Expression in Mouse Oocytes by RNA-Seq

  4. Back Matter

    Pages 253-256