mTOR

Methods and Protocols

Editors:

ISBN: 978-1-61779-429-2 (Print) 978-1-61779-430-8 (Online)

Table of contents (29 protocols)

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  1. Front Matter

    Pages i-xiii

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    Pages 1-14

    Mammalian Target of Rapamycin: A Signaling Kinase for Every Aspect of Cellular Life

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    Pages 15-28

    Biochemical and Pharmacological Inhibition of mTOR by Rapamycin and an ATP-Competitive mTOR Inhibitor

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    Pages 29-44

    Evaluation of the Nutrient-Sensing mTOR Pathway

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    Pages 45-58

    mTOR Activity Under Hypoxia

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    Pages 59-74

    Isolation of the mTOR Complexes by Affinity Purification

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    Pages 75-86

    An In Vitro Assay for the Kinase Activity of mTOR Complex 2

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    Pages 87-103

    Overexpression or Downregulation of mTOR in Mammalian Cells

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    Pages 105-124

    Detection of Cytoplasmic and Nuclear Functions of mTOR by Fractionation

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    Pages 125-169

    Evaluation of Rapamycin-Induced Cell Death

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    Pages 171-185

    Evaluation of mTOR-Regulated mRNA Translation

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    Pages 187-214

    A Genome-wide RNAi Screen for Polypeptides that Alter rpS6 Phosphorylation

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    Pages 215-225

    Immunohistochemical Analysis of mTOR Activity in Tissues

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    Pages 227-237

    Assessing Cell Size and Cell Cycle Regulation in Cells with Altered TOR Activity

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    Pages 239-250

    Quantitative Visualization of Autophagy Induction by mTOR Inhibitors

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    Pages 251-265

    The In Vivo Evaluation of Active-Site TOR Inhibitors in Models of BCR-ABL+ Leukemia

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    Pages 267-278

    Inducible raptor and rictor Knockout Mouse Embryonic Fibroblasts

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    Pages 279-293

    Expanding Human T Regulatory Cells with the mTOR-Inhibitor Rapamycin

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    Pages 295-303

    Rapamycin-Induced Enhancement of Vaccine Efficacy in Mice

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    Pages 305-316

    Utilizing a Retroviral RNAi System to Investigate In Vivo mTOR Functions in T Cells

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    Pages 317-327

    Exploring Functional In Vivo Consequences of the Selective Genetic Ablation of mTOR Signaling in T Helper Lymphocytes

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