Bone Research Protocols

Editors:

ISBN: 978-1-61779-414-8 (Print) 978-1-61779-415-5 (Online)

Table of contents (38 protocols)

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  1. Front Matter

    Pages i-xiv

  2. Culture of osteoblasts and osteocytes

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-18

      Primary Human Osteoblast Cultures

    3. No Access

      Book Protocol

      Pages 19-29

      Osteoblast Isolation from Murine Calvaria and Long Bones

    4. No Access

      Book Protocol

      Pages 31-41

      Rat Osteoblast Cultures

    5. No Access

      Book Protocol

      Pages 43-53

      Isolation of Primary Avian Osteocytes

    6. No Access

      Book Protocol

      Pages 55-66

      Isolation of Mouse Osteocytes Using Cell Fractionation for Gene Expression Analysis

    7. No Access

      Book Protocol

      Pages 67-81

      Studying Osteocyte Function Using the Cell Lines MLO-Y4 and MLO-A5

    8. No Access

      Book Protocol

      Pages 83-99

      Isolation, Differentiation, and Characterisation of Skeletal Stem Cells from Human Bone Marrow In Vitro and In Vivo

  3. Culture of osteoclasts

    1. Front Matter

      Pages 101-101

    2. No Access

      Book Protocol

      Pages 103-117

      Rodent Osteoclast Cultures

    3. No Access

      Book Protocol

      Pages 119-143

      Isolation and Culture of Primary Chicken Osteoclasts

    4. No Access

      Book Protocol

      Pages 145-158

      Isolation and Purification of Rabbit Osteoclasts

    5. No Access

      Book Protocol

      Pages 159-175

      Generation of Human Osteoclasts from Peripheral Blood

    6. No Access

      Book Protocol

      Pages 177-186

      Osteoclast Formation in Mouse Co-cultures

    7. No Access

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      Pages 187-202

      RANKL-Mediated Osteoclast Formation from Murine RAW 264.7 cells

  4. Biochemical and molecular analysis of bone cells

    1. Front Matter

      Pages 203-203

    2. No Access

      Book Protocol

      Pages 205-222

      Transfection of Osteoclasts and Osteoclast Precursors

    3. No Access

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      Pages 223-232

      Analysis of Signalling Pathways by Western Blotting and Immunoprecipitation

    4. No Access

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      Pages 233-247

      Analysis of Transcriptional Regulation in Bone Cells

    5. No Access

      Book Protocol

      Pages 249-259

      Extraction of Nucleic Acids from Bone

    6. No Access

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      Pages 261-275

      Analysis of Gene Expression in Bone by Quantitative RT/PCR

  5. Microscopical techniques

    1. Front Matter

      Pages 277-277

    2. No Access

      Book Protocol

      Pages 279-303

      Histomorphometry in Rodents

    3. No Access

      Book Protocol

      Pages 305-320

      Studying Gene Expression in Bone by In Situ Hybridization

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