Mitochondrial Bioenergetics

Methods and Protocols

Editors:

ISBN: 978-1-61779-381-3 (Print) 978-1-61779-382-0 (Online)

Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi

  2. No Access

    Book Protocol

    Pages 1-6

    Overview of Mitochondrial Bioenergetics

  3. No Access

    Book Protocol

    Pages 7-24

    Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal Cells

  4. No Access

    Book Protocol

    Pages 25-58

    High-Resolution Respirometry: OXPHOS Protocols for Human Cells and Permeabilized Fibers from Small Biopsies of Human Muscle

  5. No Access

    Book Protocol

    Pages 59-72

    High-Throughput Analysis of Mitochondrial Oxygen Consumption

  6. No Access

    Book Protocol

    Pages 73-88

    Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices

  7. No Access

    Book Protocol

    Pages 89-101

    Mitochondrial Membrane Potential (ΔΨ) Fluctuations Associated with the Metabolic States of Mitochondria

  8. No Access

    Book Protocol

    Pages 103-117

    Safranine as a Fluorescent Probe for the Evaluation of Mitochondrial Membrane Potential in Isolated Organelles and Permeabilized Cells

  9. No Access

    Book Protocol

    Pages 119-133

    Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells

  10. No Access

    Book Protocol

    Pages 135-152

    Phenomenological Kinetic and Control Analysis of Oxidative Phosphorylation in Isolated Mitochondria

  11. No Access

    Book Protocol

    Pages 153-164

    Expression of Uncoupling Proteins in a Mammalian Cell Culture System (HEK293) and Assessment of Their Protein Function

  12. No Access

    Book Protocol

    Pages 165-182

    Measurement of Proton Leak and Electron Leak in Isolated Mitochondria

  13. No Access

    Book Protocol

    Pages 183-205

    Relation Between Mitochondrial Membrane Potential and ROS Formation

  14. No Access

    Book Protocol

    Pages 207-217

    Use of a Calcium-Sensitive Electrode for Studies on Mitochondrial Calcium Transport

  15. No Access

    Book Protocol

    Pages 219-234

    Imaging Mitochondrial Calcium Signalling with Fluorescent Probes and Single or Two Photon Confocal Microscopy

  16. No Access

    Book Protocol

    Pages 235-242

    Mitochondrial Permeability Transition Pore and Calcium Handling

  17. No Access

    Book Protocol

    Pages 243-248

    Imaging of Mitochondrial pH Using SNARF-1

  18. No Access

    Book Protocol

    Pages 249-280

    Redox Equivalents and Mitochondrial Bioenergetics

  19. No Access

    Book Protocol

    Pages 281-309

    NMR Methodologies for Studying Mitochondrial Bioenergetics

  20. No Access

    Book Protocol

    Pages 311-326

    Computational Modeling of Mitochondrial Function

  21. Back Matter

    Pages 327-333