Carbohydrate Microarrays

Methods and Protocols

Editors:

ISBN: 978-1-61779-372-1 (Print) 978-1-61779-373-8 (Online)

Table of contents (28 protocols)

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  1. Front Matter

    Pages i-xv

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    Pages 1-12

    Recent Advances and Future Challenges in Glycan Microarray Technology

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    Pages 13-29

    Chemical Synthesis of Carbohydrates and Their Surface Immobilization: A Brief Introduction

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    Pages 31-56

    General Consideration on Sialic Acid Chemistry

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    Pages 57-68

    Synthesis of Azido-Functionalized Carbohydrates for the Design of Glycoconjugates

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    Pages 69-86

    Polypyrrole-Oligosaccharide Microarray for the Measurement of Biomolecular Interactions by Surface Plasmon Resonance Imaging

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    Pages 87-101

    Glycosylated Self-Assembled Monolayers for Arrays and Surface Analysis

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    Pages 103-116

    Carbohydrate Microarrays for Enzymatic Reactions and Quantification of Binding Affinities for Glycan–Protein Interactions

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    Pages 117-136

    Neoglycolipid-Based Oligosaccharide Microarray System: Preparation of NGLs and Their Noncovalent Immobilization on Nitrocellulose-Coated Glass Slides for Microarray Analyses

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    Pages 137-148

    Preparation of a Mannose-6-Phosphate Glycan Microarray Through Fluorescent Derivatization, Phosphorylation, and Immobilization of Natural High-Mannose N-Glycans and Application in Ligand Identification of P-Type Lectins

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    Pages 149-153

    Production of Fluorous-Based Microarrays with Uncharged Carbohydrates

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    Pages 155-165

    General Procedure for the Synthesis of Neoglycoproteins and Immobilization on Epoxide-Modified Glass Slides

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    Pages 167-182

    Immobilization of Polyacrylamide-Based Glycoconjugates on Solid Phase in Immunosorbent Assays

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    Pages 183-194

    Surface Plasmon Resonance Imaging Analysis of Protein Binding to a Sialoside-Based Carbohydrate Microarray

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    Pages 195-219

    Glycoarray by DNA-Directed Immobilization

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    Pages 221-229

    Fabrication of Carbohydrate Surfaces by Using Non-derivatised Oligosaccharides

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    Pages 231-240

    Polysaccharide Microarrays: Application to the Identification of Heparan Sulphate Mimetics

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    Pages 241-249

    Carbohydrate Antigen Microarrays

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    Pages 251-267

    Probing Virus–Glycan Interactions Using Glycan Microarrays

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    Pages 269-284

    MALDI-ToF MS Analysis of Glycosyltransferase Activities on Gold Surface Arrays

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    Pages 285-302

    Microarray Technology Using Glycans Extracted from Natural Sources for Serum Antibody Fluorescent Detection

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