Chemical Proteomics

Methods and Protocols

Editors:

ISBN: 978-1-61779-363-9 (Print) 978-1-61779-364-6 (Online)

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi

  2. Introduction

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-13

      Mass Spectrometry-Based Chemoproteomic Approaches

    3. No Access

      Book Protocol

      Pages 15-21

      Chemical Proteomics in Drug Discovery

  3. Small Molecules and Probe Design

    1. Front Matter

      Pages 23-23

    2. No Access

      Book Protocol

      Pages 25-38

      Compound Immobilization and Drug-Affinity Chromatography

    3. No Access

      Book Protocol

      Pages 39-54

      Affinity-Based Chemoproteomics with Small Molecule-Peptide Conjugates

    4. No Access

      Book Protocol

      Pages 55-64

      A Chemical Proteomic Probe for Detecting Dehydrogenases: Catechol Rhodanine

    5. No Access

      Book Protocol

      Pages 65-75

      Probing Proteomes with Benzophenone Photoprobes

    6. No Access

      Book Protocol

      Pages 77-95

      Biotinylated Probes for the Analysis of Protein Modification by Electrophiles

    7. No Access

      Book Protocol

      Pages 97-125

      Profiling of Methyltransferases and Other S-Adenosyl-l-Homocysteine-Binding Proteins by Capture Compound Mass Spectrometry

  4. Target Discovery and Target Validation

    1. Front Matter

      Pages 127-127

    2. No Access

      Book Protocol

      Pages 129-140

      Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC

    3. No Access

      Book Protocol

      Pages 141-155

      Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric Mass Tags

    4. No Access

      Book Protocol

      Pages 157-165

      Affinity-Based Profiling of Dehydrogenase Subproteomes

    5. No Access

      Book Protocol

      Pages 167-181

      Probing the Specificity of Protein–Protein Interactions by Quantitative Chemical Proteomics

    6. No Access

      Book Protocol

      Pages 183-204

      Fluorescence-Based Proteasome Activity Profiling

    7. No Access

      Book Protocol

      Pages 205-218

      Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein–Drug Interactions

    8. No Access

      Book Protocol

      Pages 219-229

      Monitoring Ligand Modulation of Protein–Protein Interactions by Chemical Cross-Linking and High-Mass MALDI Mass Spectrometry

    9. No Access

      Book Protocol

      Pages 231-246

      Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

  5. Ligand Discovery

    1. Front Matter

      Pages 247-247

    2. No Access

      Book Protocol

      Pages 249-263

      Ligand Discovery Using Small-Molecule Microarrays

    3. No Access

      Book Protocol

      Pages 265-271

      Working with Small Molecules: Preparing and Storing Stock Solutions and Determination of Kinetic Solubility

    4. No Access

      Book Protocol

      Pages 273-296

      A Database for Chemical Proteomics: ChEBI

    5. No Access

      Book Protocol

      Pages 297-307

      Working with Small Molecules: Rules-of-Thumb of “Drug Likeness”

  6. Back Matter

    Pages 309-313