Protein Expression in Mammalian Cells

Methods and Protocols

Editors:

ISBN: 978-1-61779-351-6 (Print) 978-1-61779-352-3 (Online)

Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi

  2. No Access

    Book Protocol

    Pages 1-12

    Why Proteins in Mammalian Cells?

  3. No Access

    Book Protocol

    Pages 13-26

    Large-Scale Transfection of Mammalian Cells

  4. No Access

    Book Protocol

    Pages 27-39

    Selection of High Expressing Mammalian Cells by Surface Display of Reporters

  5. No Access

    Book Protocol

    Pages 41-63

    Expression of a Secreted Protein in Mammalian Cells Using Baculovirus Particles

  6. No Access

    Book Protocol

    Pages 65-74

    Transfection of Difficult-to-Transfect Primary Mammalian Cells

  7. No Access

    Book Protocol

    Pages 75-92

    Stable Protein Expression in Mammalian Cells Using Baculoviruses

  8. No Access

    Book Protocol

    Pages 93-110

    Using Matrix Attachment Regions to Improve Recombinant Protein Production

  9. No Access

    Book Protocol

    Pages 111-123

    Controlling Apoptosis to Optimize Yields of Proteins from Mammalian Cells

  10. No Access

    Book Protocol

    Pages 125-135

    Post-transcriptional Regulatory Elements for Enhancing Transient Gene Expression Levels in Mammalian Cells

  11. No Access

    Book Protocol

    Pages 137-159

    Converting Monoclonal Antibodies into Fab Fragments for Transient Expression in Mammalian Cells

  12. No Access

    Book Protocol

    Pages 161-172

    Generation of High-Expressing Cells by Methotrexate Amplification of Destabilized Dihydrofolate Reductase Selection Marker

  13. No Access

    Book Protocol

    Pages 173-187

    Tools for Coproducing Multiple Proteins in Mammalian Cells

  14. No Access

    Book Protocol

    Pages 189-211

    Identification and Characterization of Protein Glycosylation Using Specific Endo- and Exoglycosidases

  15. No Access

    Book Protocol

    Pages 213-226

    Strategies for Efficient Transfection of CHO-Cells with Plasmid DNA

  16. No Access

    Book Protocol

    Pages 227-250

    Methods for Constructing Clones for Protein Expression in Mammalian Cells

  17. No Access

    Book Protocol

    Pages 251-268

    Optimizing Transient Recombinant Protein Expression in Mammalian Cells

  18. Back Matter

    Pages 269-276