Neisseria meningitidis

Advanced Methods and Protocols

Editors:

ISBN: 978-1-61779-345-5 (Print) 978-1-61779-346-2 (Online)

Table of contents (22 protocols)

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  1. Front Matter

    Pages i-xiii

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    Pages 1-20

    Neisseria meningitidis: Biology, Microbiology, and Epidemiology

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    Pages 21-35

    Classification and Pathogenesis of Meningococcal Infections

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    Pages 37-53

    Detection of Neisseria meningitidis in Cerebrospinal Fluid Using a Multiplex PCR and the Luminex Detection Technology

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    Pages 55-72

    Generating Knock-Out and Complementation Strains of Neisseria meningitidis

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    Pages 73-89

    Identification and Functional Characterization of sRNAs in Neisseria meningitidis

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    Pages 91-106

    Expression, Purification, and Crystallization of Neisserial Outer Membrane Proteins

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    Pages 107-126

    Genome-Scale Metabolic Models: Reconstruction and Analysis

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    Pages 127-141

    TMT Labelling for the Quantitative Analysis of Adaptive Responses in the Meningococcal Proteome

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    Pages 143-152

    Meningococcal Ligands and Molecular Targets of the Host

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    Pages 153-168

    In Vivo Imaging of Meningococcal Disease Dynamics

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    Pages 169-184

    Methods for Studying Neisseria meningitidisBiofilms

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    Pages 185-195

    A Laminar-Flow Chamber Assay for Measuring Bacterial Adhesion Under Shear Stress

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    Pages 197-216

    Techniques to Measure Pilus Retraction Forces

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    Pages 217-235

    Human Dendritic Cell Culture and Bacterial Infection

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    Pages 237-252

    Hydrogen–Deuterium Exchange Coupled to Mass Spectrometry to Investigate Ligand–Receptor Interactions

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    Pages 253-266

    Visualising PAMP–PRR Interactions Using Nanoscale Imaging

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    Pages 267-293

    Transcriptome Analyses in the Interaction of Neisseria meningitidis with Mammalian Host Cells

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    Pages 295-317

    The Use of the Pan-Neisseria Microarray and Experimental Design for Transcriptomics Studies of Neisseria

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    Pages 319-341

    Analysis of Parameters Associated with Prevention of Cellular Apoptosis by Pathogenic Neisseriae and Purified Porins

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    Pages 343-360

    Analysis of the Immune Response to Neisseria meningitidis Using a Proteomics Approach

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