Kinase Inhibitors

Methods and Protocols

Editors:

ISBN: 978-1-61779-336-3 (Print) 978-1-61779-337-0 (Online)

Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi

  2. No Access

    Book Protocol

    Pages 1-34

    Targeting Cancer with Small-Molecular-Weight Kinase Inhibitors

  3. No Access

    Book Protocol

    Pages 35-44

    Small-Molecule Protein and Lipid Kinase Inhibitors in Inflammation and Specific Models for Their Evaluation

  4. No Access

    Book Protocol

    Pages 45-54

    Measuring the Activity of Leucine-Rich Repeat Kinase 2: A Kinase Involved in Parkinson’s Disease

  5. No Access

    Book Protocol

    Pages 55-67

    Measuring PI3K Lipid Kinase Activity

  6. No Access

    Book Protocol

    Pages 69-81

    A Fluorescence Polarization Assay for the Discovery of Inhibitors of the Polo-Box Domain of Polo-Like Kinase 1

  7. No Access

    Book Protocol

    Pages 83-107

    Assessment of Hepatotoxicity Potential of Drug Candidate Molecules Including Kinase Inhibitors by Hepatocyte Imaging Assay Technology and Bile Flux Imaging Assay Technology

  8. No Access

    Book Protocol

    Pages 109-118

    Kinase Inhibitor Selectivity Profiling Using Differential Scanning Fluorimetry

  9. No Access

    Book Protocol

    Pages 119-134

    Chemoproteomic Characterization of Protein Kinase Inhibitors Using Immobilized ATP

  10. No Access

    Book Protocol

    Pages 135-147

    Proteome-Wide Identification of Staurosporine-Binding Kinases Using Capture Compound Mass Spectrometry

  11. No Access

    Book Protocol

    Pages 149-160

    Affinity Purification of Proteins Binding to Kinase Inhibitors Immobilized on Self-Assembling Monolayers

  12. No Access

    Book Protocol

    Pages 161-177

    Kinase Inhibitor Profiling Using Chemoproteomics

  13. No Access

    Book Protocol

    Pages 179-190

    Covalent Cross-Linking of Kinases with Their Corresponding Peptide Substrates

  14. No Access

    Book Protocol

    Pages 191-202

    Receptor Tyrosine Kinase Inhibitor Profiling Using Bead-Based Multiplex Sandwich Immunoassays

  15. No Access

    Book Protocol

    Pages 203-215

    Monitoring Phosphoproteomic Response to Targeted Kinase Inhibitors Using Reverse-Phase Protein Microarrays

  16. No Access

    Book Protocol

    Pages 217-231

    Measuring Phosphorylation-Specific Changes in Response to Kinase Inhibitors in Mammalian Cells Using Quantitative Proteomics

  17. No Access

    Book Protocol

    Pages 233-253

    Investigation of Acquired Resistance to EGFR-Targeted Therapies in Lung Cancer Using cDNA Microarrays

  18. Back Matter

    Pages 255-256