Handbook of ELISPOT

Methods and Protocols

Editors:

ISBN: 978-1-61779-324-0 (Print) 978-1-61779-325-7 (Online)

Table of contents (19 protocols)

  1. Front Matter

    Pages i-x

  2. Setting and Performing ELISPOT Assay

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-23

      Unique Strengths of ELISPOT for T Cell Diagnostics

    3. No Access

      Book Protocol

      Pages 25-36

      The Impact of Harmonization on ELISPOT Assay Performance

  3. ELISPOT for Veterinary Research

    1. Front Matter

      Pages 37-37

    2. No Access

      Book Protocol

      Pages 39-45

      Equine ELISPOT Assay to Study Secretion of IFNγ and IL-4 from Peripheral Blood Mononuclear Cells

    3. No Access

      Book Protocol

      Pages 47-63

      Utilization of Feline ELISPOT for Mapping Vaccine Epitopes

    4. No Access

      Book Protocol

      Pages 65-74

      Analyzing Cellular Immunity to AAV in a Canine Model Using ELISPOT Assay

  4. Advanced ELISPOT Techniques

    1. Front Matter

      Pages 75-75

    2. No Access

      Book Protocol

      Pages 77-85

      Dual- and Triple-Color Fluorospot

    3. No Access

      Book Protocol

      Pages 87-96

      ELISPOT Assay as a Tool to Study Oxidative Stress in Lymphocytes

    4. No Access

      Book Protocol

      Pages 97-104

      ELISPOT Assay for Neuroscience Research: Studying TNFα Secretion from Microglial Cells

    5. No Access

      Book Protocol

      Pages 105-114

      ELISPOT Assay as a Tool to Study the Effects of Stem Cells on Cytokine Secretion

    6. No Access

      Book Protocol

      Pages 115-122

      Combining ELISPOT and ELISA to Measure Amounts of Cytokines Secreted by a Single Cell

  5. Image and Data Analysis

    1. Front Matter

      Pages 123-123

    2. No Access

      Book Protocol

      Pages 125-143

      How ELISPOT Morphology Reflects on the Productivity and Kinetics of Cells’ Secretory Activity

    3. No Access

      Book Protocol

      Pages 145-153

      Mathematical Algorithms for Automatic Search, Recognition, and Detection of Spots in ELISPOT Assay

    4. No Access

      Book Protocol

      Pages 155-171

      Objective, User-Independent ELISPOT Data Analysis Based on Scientifically Validated Principles

    5. No Access

      Book Protocol

      Pages 173-183

      Statistical Analysis of ELISPOT Assays

    6. No Access

      Book Protocol

      Pages 185-196

      Response Determination Criteria for ELISPOT: Toward a Standard that Can Be Applied Across Laboratories

  6. ELISPOT Assay for Vaccine Development and Diagnostics

    1. Front Matter

      Pages 197-197

    2. No Access

      Book Protocol

      Pages 199-218

      Detection of Vaccinia Virus-Specific IFNγ and IL-10 Secretion from Human PBMCs and CD8+ T Cells by ELISPOT

    3. No Access

      Book Protocol

      Pages 219-227

      ELISPOT Assays to Enumerate Bovine IFN-γ-Secreting Cells for the Development of Novel Vaccines Against Bovine Tuberculosis

    4. No Access

      Book Protocol

      Pages 229-241

      IL-7 Addition Increases Spot Size and Number as Measured by T-SPOT.TB ®

    5. No Access

      Book Protocol

      Pages 243-256

      Overview of Membranes and Membrane Plates Used in Research and Diagnostic ELISPOT Assays

  7. Back Matter

    Pages 257-261