Epigenetics Protocols


ISBN: 978-1-61779-315-8 (Print) 978-1-61779-316-5 (Online)
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Table of contents (23 protocols)

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  1. Front Matter

    Pages i-xi

  2. Book Protocol

    Pages 1-10

    Advances in Epigenetic Technology

  3. Book Protocol

    Pages 11-21

    DNA Methylation Detection: Bisulfite Genomic Sequencing Analysis

  4. Book Protocol

    Pages 23-32

    Methylation-Specific PCR

  5. Book Protocol

    Pages 33-53

    Analysing DNA Methylation Using Bisulphite Pyrosequencing

  6. Book Protocol

    Pages 55-71

    Closed-Tube PCR Methods for Locus-Specific DNA Methylation Analysis

  7. Book Protocol

    Pages 73-88

    A Combined Bisulfite Restriction Analysis Bioinformatics Tool: Methyl-Typing

  8. Book Protocol

    Pages 89-100

    SIRPH: An HPLC-Based SNuPE for Quantitative Methylation Measurement at Specific CpG Sites

  9. Book Protocol

    Pages 101-112

    Restriction Landmark Genome Scanning

  10. Book Protocol

    Pages 113-123

    Methylated DNA Immunoprecipitation Genome-Wide Analysis

  11. Book Protocol

    Pages 125-133

    Methylated-CpG Island Recovery Assay

  12. Book Protocol

    Pages 135-144

    Global DNA Methylation Analysis Using the Luminometric Methylation Assay

  13. Book Protocol

    Pages 145-156

    Inhibition of DNA Methylation in Somatic Cells

  14. Book Protocol

    Pages 157-177

    DNA Methyltransferase Assays

  15. Book Protocol

    Pages 179-193

    A Chromatin Immunoprecipitation Protocol for Small Cell Numbers

  16. Book Protocol

    Pages 195-212

    Native Chromatin Immunoprecipitation

  17. Book Protocol

    Pages 213-223

    Q-PCR in Combination with ChIP Assays to Detect Changes in Chromatin Acetylation

  18. Book Protocol

    Pages 225-237

    Sequential Chromatin Immunoprecipitation Assay and Analysis

  19. Book Protocol

    Pages 239-251

    Combined Chromatin Immunoprecipitation and Bisulfite Methylation Sequencing Analysis

  20. Book Protocol

    Pages 253-264

    Studying RNA–Protein Interactions In Vivo By RNA Immunoprecipitation

  21. Book Protocol

    Pages 265-286

    Using ChIP-Seq Technology to Generate High-Resolution Profiles of Histone Modifications

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