Epigenetics Protocols

Editors:

ISBN: 978-1-61779-315-8 (Print) 978-1-61779-316-5 (Online)

Table of contents (23 protocols)

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  1. Front Matter

    Pages i-xi

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    Pages 1-10

    Advances in Epigenetic Technology

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    Pages 11-21

    DNA Methylation Detection: Bisulfite Genomic Sequencing Analysis

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    Pages 23-32

    Methylation-Specific PCR

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    Pages 33-53

    Analysing DNA Methylation Using Bisulphite Pyrosequencing

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    Pages 55-71

    Closed-Tube PCR Methods for Locus-Specific DNA Methylation Analysis

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    Pages 73-88

    A Combined Bisulfite Restriction Analysis Bioinformatics Tool: Methyl-Typing

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    Pages 89-100

    SIRPH: An HPLC-Based SNuPE for Quantitative Methylation Measurement at Specific CpG Sites

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    Pages 101-112

    Restriction Landmark Genome Scanning

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    Pages 113-123

    Methylated DNA Immunoprecipitation Genome-Wide Analysis

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    Pages 125-133

    Methylated-CpG Island Recovery Assay

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    Pages 135-144

    Global DNA Methylation Analysis Using the Luminometric Methylation Assay

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    Pages 145-156

    Inhibition of DNA Methylation in Somatic Cells

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    Pages 157-177

    DNA Methyltransferase Assays

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    Pages 179-193

    A Chromatin Immunoprecipitation Protocol for Small Cell Numbers

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    Pages 195-212

    Native Chromatin Immunoprecipitation

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    Pages 213-223

    Q-PCR in Combination with ChIP Assays to Detect Changes in Chromatin Acetylation

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    Pages 225-237

    Sequential Chromatin Immunoprecipitation Assay and Analysis

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    Book Protocol

    Pages 239-251

    Combined Chromatin Immunoprecipitation and Bisulfite Methylation Sequencing Analysis

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    Pages 253-264

    Studying RNA–Protein Interactions In Vivo By RNA Immunoprecipitation

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    Pages 265-286

    Using ChIP-Seq Technology to Generate High-Resolution Profiles of Histone Modifications

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