Neuropeptides

Methods and Protocols

Editors:

ISBN: 978-1-61779-309-7 (Print) 978-1-61779-310-3 (Online)

Table of contents (25 protocols)

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  1. Front Matter

    Pages i-xi

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    Pages 1-36

    What Are Neuropeptides?

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    Pages 37-56

    Neuropeptide Localization in Nonmammalian Vertebrates

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    Pages 57-71

    Combined Light and Electron Microscopic Visualization of Neuropeptides and Their Receptors in Central Neurons

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    Pages 73-87

    Neuropeptide RNA Localization in Tissue Sections

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    Pages 89-110

    Intron-Specific Neuropeptide Probes

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    Pages 111-126

    Direct In Situ RT-PCR

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    Pages 127-135

    Laser Capture Microdissection and Quantitative-PCR Analysis

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    Pages 137-145

    Visualization of Peptide Secretory Vesicles in Living Nerve Cells

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    Pages 147-170

    Fluorescence Imaging with Single-Molecule Sensitivity and Fluorescence Correlation Spectroscopy of Cell-Penetrating Neuropeptides

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    Pages 171-180

    Analysis of Neuroimmune Interactions by an In Vitro Coculture Approach

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    Pages 181-189

    Electrophysiology

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    Pages 191-201

    Localization of Neuropeptides by Radioimmunoassay

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    Pages 203-221

    Reversed-Phase HPLC and Hyphenated Analytical Strategies for Peptidomics

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    Pages 223-236

    Neuropeptidomics: Mass Spectrometry-Based Qualitative and Quantitative Analysis

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    Pages 237-259

    Suppression Subtractive Hybridization

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    Pages 261-269

    Neuropeptide Microdialysis in Free-Moving Animals

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    Pages 271-285

    Antibody Microprobes for Detecting Neuropeptide Release

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    Pages 287-294

    Neuropeptidases

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    Pages 295-302

    Neuropeptide Autoantibodies Assay

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    Pages 303-312

    Intranasal Delivery of Neuropeptides

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