Molecular Chaperones

Methods and Protocols

Editors:

ISBN: 978-1-61779-294-6 (Print) 978-1-61779-295-3 (Online)

Table of contents (23 protocols)

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  1. Front Matter

    Pages i-xviii

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    Pages 1-20

    Targeted Deletion of Hsf1, 2, and 4 Genes in Mice

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    Pages 21-32

    The Role of Heat Shock Factors in Stress-Induced Transcription

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    Pages 33-44

    Hsp90 and Client Protein Maturation

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    Pages 45-66

    The Role of p23, Hop, Immunophilins, and Other Co-chaperones in Regulating Hsp90 Function

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    Pages 67-74

    Detecting HSP90 Phosphorylation

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    Pages 75-81

    Role of Molecular Chaperones in Biogenesis of the Protein Kinome

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    Pages 83-91

    Nucleotide Exchange Factors for Hsp70 Chaperones

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    Pages 93-103

    Reconstitution of CHIP E3 Ubiquitin Ligase Activity

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    Pages 105-119

    Structure–Functions of HspB1 (Hsp27)

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    Pages 121-136

    Combined Lentiviral and RNAi Technologies for the Delivery and Permanent Silencing of the hsp25 Gene

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    Pages 137-143

    Quantification of HSP27 and HSP70 Molecular Chaperone Activities

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    Pages 145-153

    Measuring Hsp72 (HSPA1A) by Indirect Sandwich ELISA

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    Pages 155-164

    Analysis of Heat-Shock Protein Localisation Using Flow Cytometry

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    Pages 165-188

    Quantitation of Heat-Shock Proteins in Clinical Samples Using Mass Spectrometry

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    Pages 189-203

    Bioinformatic Approach to Identify Chaperone Pathway Relationship from Large-Scale Interaction Networks

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    Pages 205-230

    Hsp70: Anti-apoptotic and Tumorigenic Protein

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    Pages 231-244

    Determination of Cell Survival or Death

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    Pages 245-254

    Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer

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    Pages 255-265

    Preparation of a Heat-Shock Protein 70-Based Vaccine from DC–Tumor Fusion Cells

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    Pages 267-275

    Isolation of Heat Shock Protein Complexes

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