Poly(ADP-ribose) Polymerase

Methods and Protocols

Editors:

ISBN: 978-1-61779-269-4 (Print) 978-1-61779-270-0 (Online)

Table of contents (30 protocols)

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  1. Front Matter

    Pages i-xv

  2. Biochemical Approaches, In Vitro and In Vivo

    1. Front Matter

      Pages 1-1

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      Pages 3-34

      Approaches to Detect PARP-1 Activation In Vivo, In Situ, and In Vitro

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      Pages 35-46

      Quantification of Poly(ADP-Ribose) In Vitro: Determination of the ADP-Ribose Chain Length and Branching Pattern

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      Pages 47-55

      Assay for Protein Modification by Poly-ADP-Ribose In Vitro

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      Pages 57-66

      Identification of Distinct Amino Acids as ADP-Ribose Acceptor Sites by Mass Spectrometry

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      Pages 67-82

      Noncovalent Protein Interaction with Poly(ADP-Ribose)

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      Pages 83-91

      Noncovalent pADPr Interaction with Proteins and Competition with RNA for Binding to Proteins

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      Pages 93-115

      Affinity-Based Assays for the Identification and Quantitative Evaluation of Noncovalent Poly(ADP-Ribose)-Binding Proteins

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      Pages 117-134

      Detecting and Quantifying pADPr In Vivo

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      Pages 135-152

      Purification of Recombinant Poly(ADP-Ribose) Polymerases

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      Pages 153-164

      Methods for Purification of Proteins Associated with Cellular Poly(ADP-Ribose) and PARP-Specific Poly(ADP-Ribose)

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      Pages 165-190

      Purification and Characterization of Poly(ADP-Ribosyl)ated DNA Replication/Repair Complexes

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      Pages 191-208

      PARP1 Genomics: Chromatin Immunoprecipitation Approach Using Anti-PARP1 Antibody (ChIP and ChIP-seq)

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      Pages 209-226

      Purification of Human PARP-1 and PARP-1 Domains from Escherichia coli for Structural and Biochemical Analysis

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      Pages 227-236

      Regulation of Chromatin Structure by PARP-1

  3. Genetic Manipulation Approaches

    1. Front Matter

      Pages 237-237

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      Pages 239-266

      The Use of PARP Inhibitors in Cancer Therapy: Use as Adjuvant with Chemotherapy or Radiotherapy; Use as a Single Agent in Susceptible Patients; Techniques Used to Identify Susceptible Patients

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      Pages 267-275

      Quantification of PARP Activity in Human Tissues: Ex Vivo Assays in Blood Cells and Immunohistochemistry in Human Biopsies

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      Pages 277-291

      Assays for Hypermethylation of the BRCA1 Gene Promoter in Tumor Cells to Predict Sensitivity to PARP-Inhibitor Therapy

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      Pages 293-312

      Generation of Poly(ADP-ribosyl)ation Deficient Mutants of the Transcription Factor, CTCF

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      Pages 313-336

      Phenotypic Characterization of Parp-1 and Parp-2 Deficient Mice and Cells

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      Pages 337-348

      Poly(ADP-ribosyl)ation Pathways in Mammals: The Advantage of Murine PARG Null Mutation

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