Human Embryonic and Induced Pluripotent Stem Cells

Lineage-Specific Differentiation Protocols

Editors:

ISBN: 978-1-61779-266-3 (Print) 978-1-61779-267-0 (Online)

Table of contents (34 protocols)

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  1. Front Matter

    Pages i-xvi

  2. Maintenance and Cryopreservation of ES and iPS cells

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-12

      Feeder-Free Growth of Undifferentiated Human Embryonic Stem Cells

    3. No Access

      Book Protocol

      Pages 13-24

      Growth of Human Embryonic Stem Cells in Long-Term Hypoxia

    4. No Access

      Book Protocol

      Pages 25-35

      Laboratory-Scale Purification of a Recombinant E-Cadherin-IgG Fc Fusion Protein That Provides a Cell Surface Matrix for Extended Culture and Efficient Subculture of Human Pluripotent Stem Cells

    5. No Access

      Book Protocol

      Pages 37-50

      Scale-Up of Single Cell–Inoculated Suspension Cultures of Human Embryonic Stem Cells

    6. No Access

      Book Protocol

      Pages 51-58

      Three-Dimensional Culture for Expansion and Differentiation of Embryonic Stem Cells

    7. No Access

      Book Protocol

      Pages 59-69

      Expansion of Pluripotent Stem Cells in Defined, Xeno-Free Culture System

    8. No Access

      Book Protocol

      Pages 71-83

      Pluripotent Stem Cells In Vitro from Human Primordial Germ Cells

    9. No Access

      Book Protocol

      Pages 85-90

      Cryopreservation of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells

  3. Generation of Human Induced Pluripotent Stem Cells: Viral and Nonviral Vector-Based Nuclear Reprogramming

    1. Front Matter

      Pages 91-91

    2. No Access

      Book Protocol

      Pages 93-111

      Induced Pluripotent Stem Cells (iPSC) from Cord Blood CD133+ Cells Using Oct4 and Sox2

    3. No Access

      Book Protocol

      Pages 113-131

      Generation, Maintenance, and Differentiation of Human iPS Cells from Cord Blood

    4. No Access

      Book Protocol

      Pages 133-149

      Generation of iPS Cells from Human Umbilical Vein Endothelial Cells by Lentiviral Transduction and Their Differentiation to Neuronal Lineage

    5. No Access

      Book Protocol

      Pages 151-160

      Generation of Human Induced Pluripotent Stem Cells from Endoderm Origin Cells

    6. No Access

      Book Protocol

      Pages 161-171

      Derivation of Human Induced Pluripotent Stem Cells on Autologous Feeders

    7. No Access

      Book Protocol

      Pages 173-190

      Human Mesenchymal Stem Cells and iPS Cells (Preparation Methods)

    8. No Access

      Book Protocol

      Pages 191-201

      Retroviral Vector-Based Approaches for the Generation of Human Induced Pluripotent Stem Cells from Fibroblasts and Keratinocytes

    9. No Access

      Book Protocol

      Pages 203-227

      Generation of Nonviral Integration-Free Induced Pluripotent Stem Cells from Plucked Human Hair Follicles

  4. Generation of Patient-Specific iPS Cells for Clinical Application

    1. Front Matter

      Pages 229-229

    2. No Access

      Book Protocol

      Pages 231-247

      Generation of iPS Cells from Human Skin Biopsy

    3. No Access

      Book Protocol

      Pages 249-264

      Generation of Induced Pluripotent Stem Cells from Human Amnion Cells

  5. Lineage-Specific Differentiation of hES and iPS Cells

    1. Front Matter

      Pages 265-265

    2. No Access

      Book Protocol

      Pages 267-275

      In Vitro Two-Dimensional Endothelial Differentiation of Human Embryonic Stem Cells

    3. No Access

      Book Protocol

      Pages 277-294

      Feeder-Free Culture for High Efficiency Production of Subculturable Vascular Endothelial Cells from Human Embryonic Stem Cells

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