Androgen Action

Methods and Protocols

Editors:

ISBN: 978-1-61779-242-7 (Print) 978-1-61779-243-4 (Online)

Table of contents (22 protocols)

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  1. Front Matter

    Pages i-xi

  2. Reviews

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-24

      Molecular Mechanisms of Androgen Action – A Historical Perspective

    3. Book Protocol

      Pages 25-44

      Androgen Action During Prostate Carcinogenesis

  3. Analysis of Androgens

    1. Front Matter

      Pages 45-45

    2. No Access

      Book Protocol

      Pages 47-57

      Androgen Quantitation in Prostate Cancer Tissue Using Liquid Chromatography Tandem Mass Spectrometry

    3. No Access

      Book Protocol

      Pages 59-68

      Ligand Competition Binding Assay for the Androgen Receptor

  4. Androgen receptor biology

    1. Front Matter

      Pages 69-69

    2. No Access

      Book Protocol

      Pages 71-80

      Analysis of Androgen Receptor Activity by Reporter Gene Assays

    3. No Access

      Book Protocol

      Pages 81-93

      Identification and Characterization of Androgen Response Elements

    4. No Access

      Book Protocol

      Pages 95-112

      In Vitro and In Vivo Silencing of the Androgen Receptor

    5. No Access

      Book Protocol

      Pages 113-129

      Analysis of Interdomain Interactions of the Androgen Receptor

    6. No Access

      Book Protocol

      Pages 131-145

      Methods to Study Dynamic Interaction of Androgen Receptor with Chromatin in Living Cells

    7. No Access

      Book Protocol

      Pages 147-166

      FRET Analysis of Androgen Receptor Structure and Biochemistry in Living Cells

  5. Analysis of Androgen Receptor Modifications and Interactions

    1. Front Matter

      Pages 167-167

    2. No Access

      Book Protocol

      Pages 169-181

      Analysis of Nuclear Receptor Acetylation

    3. No Access

      Book Protocol

      Pages 183-197

      Analysis of Androgen Receptor SUMOylation

    4. No Access

      Book Protocol

      Pages 199-223

      Analysis of Ligand-Specific Co-repressor Binding to the Androgen Receptor

    5. No Access

      Book Protocol

      Pages 225-251

      Detection of Ligand-Selective Interactions of the Human Androgen Receptor by SELDI-MS-TOF

  6. Recent Advances in Studying Androgen Receptor Signalling

    1. Front Matter

      Pages 253-253

    2. No Access

      Book Protocol

      Pages 255-273

      Global Identification of Androgen Response Elements

    3. No Access

      Book Protocol

      Pages 275-293

      Tissue-Specific Knockout of Androgen Receptor in Mice

    4. No Access

      Book Protocol

      Pages 295-312

      Methodology to Investigate Androgen-Sensitive and Castration-Resistant Human Prostate Cancer Xenografts in Preclinical Setting

    5. No Access

      Book Protocol

      Pages 313-331

      Automated Microscopy and Image Analysis for Androgen Receptor Function

  7. Cross-talk of Androgen and Other Signalling Pathways

    1. Front Matter

      Pages 333-333

    2. No Access

      Book Protocol

      Pages 335-348

      Androgen Regulation of ETS Gene Fusion Transcripts in Prostate Cancer

    3. No Access

      Book Protocol

      Pages 349-360

      Regulation of Apoptosis by Androgens in Prostate Cancer Cells

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