Cell Migration

Developmental Methods and Protocols

Editors:

ISBN: 978-1-61779-206-9 (Print) 978-1-61779-207-6 (Online)

Table of contents (30 protocols)

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  1. Front Matter

    Pages i-xii

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    Pages 1-24

    Cell Migration: An Overview

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    Pages 25-30

    Scratch-Wound Assay

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    Pages 31-40

    HGF-Induced DU145 Cell Scatter Assay

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    Pages 41-51

    Using the Dunn Chemotaxis Chamber to Analyze Primary Cell Migration in Real Time

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    Pages 53-64

    Imaging Cells Within 3D Cell-Derived Matrix

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    Pages 65-86

    Live Cell Imaging of Neuronal Growth Cone Motility and Guidance In Vitro

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    Pages 87-95

    Boyden Chamber

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    Pages 97-110

    Transwell® Invasion Assays

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    Pages 111-136

    Imaging Podosome Dynamics and Matrix Degradation

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    Pages 137-147

    Endothelial Cell Migration Under Flow

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    Pages 149-165

    In Vitro Analysis of Chemotactic Leukocyte Migration in 3D Environments

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    Pages 167-190

    Quantification of Transendothelial Migration Using Three-Dimensional Confocal Microscopy

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    Pages 191-203

    Chemotaxis of Slow Migrating Mammalian Cells Analysed by Video Microscopy

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    Pages 205-222

    Live Cell Fluorescence Microscopy Techniques

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    Pages 223-232

    Measuring Invasion in an Organotypic Model

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    Pages 233-247

    Analysis of Cell Migration Using Caenorhabditis elegans as a Model System

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    Pages 249-260

    Drosophila Hemocyte Migration: An In Vivo Assay for Directional Cell Migration

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    Pages 261-275

    Measuring Inflammatory Cell Migration in the Zebrafish

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    Pages 277-286

    Border Cell Migration: A Model System for Live Imaging and Genetic Analysis of Collective Cell Movement

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    Pages 287-309

    Assessment of Development and Chemotaxis in Dictyostelium discoideum Mutants

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