Laser Capture Microdissection

Methods and Protocols

Editors:

ISBN: 978-1-61779-162-8 (Print) 978-1-61779-163-5 (Online)

Table of contents (40 protocols)

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  1. Front Matter

    Pages i-xvii

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    Pages 1-15

    Laser Capture Microdissection: Methods and Applications

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    Pages 17-45

    Laser Microdissection for Gene Expression Profiling

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    Pages 47-56

    Gene Expression Using the PALM System

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    Pages 57-66

    Immunoguided Microdissection Techniques

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    Pages 67-75

    Optimized RNA Extraction from Non-deparaffinized, Laser-Microdissected Material

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    Pages 77-84

    Laser Capture Microdissection for Analysis of Gene Expression in Formalin-Fixed Paraffin-Embedded Tissue

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    Pages 85-94

    MicroRNA Profiling Using RNA from Microdissected Immunostained Tissue

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    Pages 95-106

    Profiling Solid Tumor Heterogeneity by LCM and Biological MS of Fresh-Frozen Tissue Sections

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    Pages 107-118

    Amplification Testing in Breast Cancer by Multiplex Ligation-Dependent Probe Amplification of Microdissected Tissue

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    Pages 119-142

    Detection and Quantification of MicroRNAs in Laser-Microdissected Formalin-Fixed Paraffin-Embedded Breast Cancer Tissues

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    Pages 143-154

    Laser Capture Microdissection Applications in Breast Cancer Proteomics

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    Pages 155-163

    Proteomic Analysis of Laser Microdissected Ovarian Cancer Tissue with SELDI-TOF MS

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    Pages 165-180

    LCM Assisted Biomarker Discovery from Archival Neoplastic Gastrointestinal Tissues

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    Pages 181-187

    Purification of Diseased Cells from Barrett’s Esophagus and Related Lesions by Laser Capture Microdissection

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    Pages 189-196

    Laser Microdissection of Intestinal Epithelial Cells and Downstream Analysis

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    Pages 197-202

    Application of Laser Microdissection and Quantitative PCR to Assess the Response of Esophageal Cancer to Neoadjuvant Chemo-Radiotherapy

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    Pages 203-221

    Oligonucleotide Microarray Expression Profiling of Contrasting Invasive Phenotypes in Colorectal Cancer

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    Pages 223-232

    Evaluation of Gastrointestinal mtDNA Depletion in Mitochondrial Neurogastrointestinal Encephalomyopathy (MNGIE)

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    Pages 233-244

    Laser Microdissection for Gene Expression Study of Hepatocellular Carcinomas Arising in Cirrhotic and Non-Cirrhotic Livers

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    Pages 245-256

    Laser Capture Microdissection of Pancreatic Ductal Adeno-Carcinoma Cells to Analyze EzH2 by Western Blot Analysis

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