DNA Nanotechnology

Methods and Protocols

Editors:

ISBN: 978-1-61779-141-3 (Print) 978-1-61779-142-0 (Online)

Table of contents (22 protocols)

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  1. Front Matter

    Pages i-xii

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    Book Protocol

    Pages 1-11

    Synthesis and Characterization of Self-Assembled DNA Nanostructures

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    Pages 13-32

    Protocols for Self-Assembly and Imaging of DNA Nanostructures

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    Pages 33-47

    Self-Assembly of Metal-DNA Triangles and DNA Nanotubes with Synthetic Junctions

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    Pages 49-59

    DNA-Templated Pd Conductive Metallic Nanowires

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    Pages 61-77

    A Method to Map Spatiotemporal pH Changes Inside Living Cells Using a pH-Triggered DNA Nanoswitch

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    Pages 79-92

    Control of Helical Handedness in DNA and PNA Nanostructures

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    Pages 93-104

    G-Quartet, G-Quadruplex, and G-Wire Regulated by Chemical Stimuli

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    Pages 105-113

    Preparation and Atomic Force Microscopy of Quadruplex DNA

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    Pages 115-140

    Synthesis of Long DNA-Based Nanowires

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    Pages 141-150

    G-Wire Synthesis and Modification with Gold Nanoparticle

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    Pages 151-168

    Preparation of DNA Nanostructures with Repetitive Binding Motifs by Rolling Circle Amplification

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    Pages 169-185

    Controlled Confinement of DNA at the Nanoscale: Nanofabrication and Surface Bio-Functionalization

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    Pages 187-197

    Templated Assembly of DNA Origami Gold Nanoparticle Arrays on Lithographically Patterned Surfaces

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    Pages 199-207

    DNA-Modified Single Crystal and Nanoporous Silicon

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    Pages 209-221

    The Atomic Force Microscopy as a Lithographic Tool: Nanografting of DNA Nanostructures for Biosensing Applications

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    Pages 223-234

    Trapping and Immobilization of DNA Molecules Between Nanoelectrodes

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    Pages 235-254

    DNA Contour Length Measurements as a Tool for the Structural Analysis of DNA and Nucleoprotein Complexes

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    Pages 255-271

    DNA Molecular Handles for Single-Molecule Protein-Folding Studies by Optical Tweezers

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    Book Protocol

    Pages 273-289

    Optimal Practices for Surface-Tethered Single Molecule Total Internal Reflection Fluorescence Resonance Energy Transfer Analysis

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    Book Protocol

    Pages 291-303

    Engineering Mononucleosomes for Single-Pair FRET Experiments

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