Immune Receptors

Methods and Protocols

Editors:

ISBN: 978-1-61779-138-3 (Print) 978-1-61779-139-0 (Online)

Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi

  2. No Access

    Book Protocol

    Pages 1-19

    Screening for Ligands of C-Type Lectin-Like Receptors

  3. No Access

    Book Protocol

    Pages 21-33

    Yeast Surface Display of Lamprey Variable Lymphocyte Receptors

  4. No Access

    Book Protocol

    Pages 35-50

    Identification of Scavenger Receptor Ligands

  5. No Access

    Book Protocol

    Pages 51-67

    Construction, Expression, and Purification of Chimeric Protein Reagents Based on Immunoglobulin Fc Regions

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    Book Protocol

    Pages 69-82

    Innate Immune Receptors for Nucleic Acids

  7. No Access

    Book Protocol

    Pages 83-106

    Analysis of Receptor–Ligand Interactions by Surface Plasmon Resonance

  8. No Access

    Book Protocol

    Pages 107-119

    Cell-Based Reporter Assay to Analyze Activation of Nod1 and Nod2

  9. No Access

    Book Protocol

    Pages 121-132

    Determining FcεRI Diffusional Dynamics via Single Quantum Dot Tracking

  10. No Access

    Book Protocol

    Pages 133-142

    Ratiometric Analysis of Subcellular Recruitment of Fc Receptors During Phagocytosis

  11. No Access

    Book Protocol

    Pages 143-153

    Assessment of the Recycling of the Membrane-Bound Chemokine, CX3CL1

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    Book Protocol

    Pages 155-167

    Measuring Immune Receptor Mobility by Fluorescence Recovery After Photobleaching

  13. No Access

    Book Protocol

    Pages 169-182

    Probing the Plasma Membrane Structure of Immune Cells Through the Analysis of Membrane Sheets by Electron Microscopy

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    Book Protocol

    Pages 183-193

    Rapamycin-Based Inducible Translocation Systems for Studying Phagocytosis

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    Book Protocol

    Pages 195-207

    Micropatterned Ligand Arrays to Study Spatial Regulation in Fc Receptor Signaling

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    Book Protocol

    Pages 209-225

    CELLISA: Reporter Cell-Based Immunization and Screening of Hybridomas Specific for Cell Surface Antigens

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    Book Protocol

    Pages 227-241

    Use of Colloidal Silica-Beads for the Isolation of Cell-Surface Proteins for Mass Spectrometry-Based Proteomics

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    Book Protocol

    Pages 243-254

    Transfection-Based Genomic Readout for Identifying Rare Transcriptional Splice Variants

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    Book Protocol

    Pages 255-271

    Characterizing Somatic Hypermutation and Gene Conversion in the Chicken DT40 Cell System

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    Book Protocol

    Pages 273-298

    Characterizing Immune Receptors from New Genome Sequences

  21. Back Matter

    Pages 299-301