Cystic Fibrosis

Diagnosis and Protocols, Volume II: Methods and Resources to Understand Cystic Fibrosis

Editors:

ISBN: 978-1-61779-119-2 (Print) 978-1-61779-120-8 (Online)

Table of contents (21 protocols)

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  1. Front Matter

    Pages i-xiv

  2. Pathophysiology Of Cystic Fibrosis

    1. Front Matter

      Pages 1-1

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      Pages 3-14

      Introduction to Section I: Overview of Approaches to Study Cystic Fibrosis Pathophysiology

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      Pages 15-33

      Imaging CFTR Protein Localization in Cultured Cells and Tissues

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      Pages 35-50

      CFTR Regulation of Epithelial Sodium Channel

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      Pages 51-76

      Methods for Evaluating Inflammation in Cystic Fibrosis

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      Pages 77-92

      Methods for ASL Measurements and Mucus Transport Rates in Cell Cultures

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      Pages 93-112

      Measurement of Fluid Secretion from Intact Airway Submucosal Glands

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      Pages 113-126

      Measurements of Intracellular Calcium Signals in Polarized Primary Cultures of Normal and Cystic Fibrosis Human Airway Epithelia

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      Pages 127-141

      Identification and Quantification of Mucin Expression

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      Pages 143-171

      Methods to Classify Bacterial Pathogens in Cystic Fibrosis

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      Pages 173-185

      Approaches to Study Differentiation and Repair of Human Airway Epithelial Cells

  3. Omic Approaches To Study Cystic Fibrosis

    1. Front Matter

      Pages 187-187

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      Pages 189-191

      Introduction to Section II: Omics in the Biology of Cystic Fibrosis

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      Pages 193-212

      Microarray mRNA Expression Profiling to Study Cystic Fibrosis

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      Pages 213-225

      Quantitative Differential Proteomics of Cystic Fibrosis Cell Models by SILAC (Stable Isotope Labelling in Cell Culture)

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      Pages 227-247

      Application of Mass Spectrometry to Study Proteomics and Interactomics in Cystic Fibrosis

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      Pages 249-264

      Functional Genomics Assays to Study CFTR Traffic and ENaC Function

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      Pages 265-278

      New Lipidomic Approaches in Cystic Fibrosis

  4. Resources

    1. Front Matter

      Pages 279-279

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      Pages 281-283

      Introduction to Section III: Resources for CFTR Research

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      Pages 285-310

      Primary Epithelial Cell Models for Cystic Fibrosis Research

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      Pages 311-334

      Comparative Biology of Cystic Fibrosis Animal Models

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      Pages 335-353

      CFTR Folding Consortium: Methods Available for Studies of CFTR Folding and Correction

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