Mammalian Cell Viability

Methods and Protocols

Editors:

ISBN: 978-1-61779-107-9 (Print) 978-1-61779-108-6 (Online)

Table of contents (21 protocols)

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  1. Front Matter

    Pages i-xi

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    Pages 1-6

    Cell Viability Assays: Introduction

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    Pages 7-12

    Cell Viability Analysis Using Trypan Blue: Manual and Automated Methods

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    Pages 13-19

    Estimation of Cell Number Based on Metabolic Activity: The MTT Reduction Assay

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    Pages 21-25

    WST-8 Analysis of Cell Viability During Osteogenesis of Human Mesenchymal Stem Cells

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    Pages 27-32

    Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye

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    Pages 33-43

    The xCELLigence System for Real-Time and Label-Free Monitoring of Cell Viability

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    Pages 45-56

    Analysis of Tumor and Endothelial Cell Viability and Survival Using Sulforhodamine B and Clonogenic Assays

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    Pages 57-63

    Annexin V/7-AAD Staining in Keratinocytes

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    Pages 65-79

    Measurement of Caspase Activity: From Cell Populations to Individual Cells

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    Pages 81-89

    Rapid Quantification of Cell Viability and Apoptosis in B-Cell Lymphoma Cultures Using Cyanine SYTO Probes

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    Pages 91-101

    Multiplexing Cell Viability Assays

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    Pages 103-114

    Cytotoxicity Testing: Measuring Viable Cells, Dead Cells, and Detecting Mechanism of Cell Death

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    Pages 115-126

    Fluorescein Diacetate for Determination of Cell Viability in 3D Fibroblast–Collagen–GAG Constructs

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    Pages 127-140

    Confocal Imaging Protocols for Live/Dead Staining in Three-Dimensional Carriers

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    Pages 141-148

    Viability Assessment of Osteocytes Using Histological Lactate Dehydrogenase Activity Staining on Human Cancellous Bone Sections

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    Pages 149-156

    Measuring Glutamate Receptor Activation-Induced Apoptotic Cell Death in Ischemic Rat Retina Using the TUNEL Assay

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    Pages 157-163

    Exploiting the Liberation of Zn2+ to Measure Cell Viability

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    Pages 165-178

    Noninvasive Bioluminescent Quantification of Viable Stem Cells in Engineered Constructs

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    Pages 179-189

    Raman Micro-Spectroscopy as a Non-invasive Cell Viability Test

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    Pages 191-208

    Closed Ampoule Isothermal Microcalorimetry for Continuous Real-Time Detection and Evaluation of Cultured Mammalian Cell Activity and Responses

  22. Back Matter

    Pages 231-240

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