Atomic Force Microscopy in Biomedical Research

Methods and Protocols

Editors:

ISBN: 978-1-61779-104-8 (Print) 978-1-61779-105-5 (Online)

Table of contents (29 protocols)

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  1. Front Matter

    Pages i-xiv

  2. The Basics of Atomic Force Microscopy

    1. Front Matter

      Pages 1-1

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      Pages 3-18

      How the Atomic Force Microscope Works?

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      Pages 19-29

      Measurement Methods in Atomic Force Microscopy

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      Pages 31-43

      Recognizing and Avoiding Artifacts in Atomic Force Microscopy Imaging

  3. Molecule Imaging

    1. Front Matter

      Pages 45-45

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      Pages 47-60

      Imaging the Spatial Orientation of Subunits Within Membrane Receptors by Atomic Force Microscopy

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      Pages 61-79

      High Resolution Imaging of Immunoglobulin G Antibodies and Other Biomolecules Using Amplitude Modulation Atomic Force Microscopy in Air

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      Pages 81-95

      Atomic Force Microscopy of Ex Vivo Amyloid Fibrils

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      Pages 97-107

      Studying Collagen Self-Assembly by Time-Lapse High-Resolution Atomic Force Microscopy

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      Pages 109-115

      Atomic Force Microscopy Imaging of Human Metaphase Chromosomes in Liquid

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      Pages 117-132

      Atomic Force Microscopy of Proteasome Assemblies

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      Pages 133-151

      Atomic Force Microscopy of Isolated Mitochondria

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      Pages 153-167

      Imaging and Interrogating Native Membrane Proteins Using the Atomic Force Microscope

  4. Nanoscale Surface Analysis and Cell Imaging

    1. Front Matter

      Pages 169-169

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      Pages 171-195

      Atomic Force Microscopy Investigation of Viruses

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      Pages 197-210

      Determination of the Kinetic On- and Off-Rate of Single Virus–Cell Interactions

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      Pages 211-221

      Atomic Force Microscopy as a Tool for the Study of the Ultrastructure of Trypanosomatid Parasites

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      Pages 223-241

      Normal and Pathological Erythrocytes Studied by Atomic Force Microscopy

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      Pages 243-257

      The Growth Cones of Living Neurons Probed by the Atomic Force Microscope

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      Pages 259-284

      Highlights on Ultrastructural Pathology of Human Sperm

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      Pages 285-300

      High-Speed Atomic Force Microscopy and Biomolecular Processes

  5. Non-Topographical Applications (Force-Spectroscopy)

    1. Front Matter

      Pages 301-301

    2. No Access

      Book Protocol

      Pages 303-329

      Atomic Force Microscopy in Mechanobiology: Measuring Microelastic Heterogeneity of Living Cells

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      Pages 331-353

      Force-Clamp Measurements of Receptor–Ligand Interactions

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