Microbial Toxins

Methods and Protocols

Editors:

ISBN: 978-1-61779-101-7 (Print) 978-1-61779-102-4 (Online)

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi

  2. Bacterial Protein Toxins

    1. Front Matter

      Pages 1-1

    2. No Access

      Book Protocol

      Pages 3-12

      Detection of Bacterial Protein Toxins by Solid Phase Magnetic Immunocapture and Mass Spectrometry

    3. No Access

      Book Protocol

      Pages 13-22

      Sensitive and Rapid Detection of Cholera Toxin-Producing Vibrio cholerae Using Loop-Mediated Isothermal Amplification

    4. No Access

      Book Protocol

      Pages 23-36

      Ultrasensitive Detection of Botulinum Neurotoxins and Anthrax Lethal Factor in Biological Samples by ALISSA

    5. No Access

      Book Protocol

      Pages 37-48

      Examination of Bacillus anthracis Spores by Multiparameter Flow Cytometry

    6. No Access

      Book Protocol

      Pages 49-59

      A Cell-Based Fluorescent Assay to Detect the Activity of Shiga Toxin and Other Toxins that Inhibit Protein Synthesis

    7. No Access

      Book Protocol

      Pages 61-71

      Use of a Vero Cell-Based Fluorescent Assay to Assess Relative Toxicities of Shiga Toxin 2 Subtypes from Escherichia coli

    8. No Access

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      Pages 73-86

      Molecular Methods: Chip Assay and Quantitative Real-Time PCR: In Detecting Hepatotoxic Cyanobacteria

  3. Endotoxins

    1. Front Matter

      Pages 87-87

    2. No Access

      Book Protocol

      Pages 89-99

      Capillary Electrophoresis Chips for Fingerprinting Endotoxin Chemotypes from Whole-Cell Lysates

    3. No Access

      Book Protocol

      Pages 101-112

      Isolation of Smooth-Type Lipopolysaccharides to Electrophoretic Homogeneity

    4. No Access

      Book Protocol

      Pages 113-122

      A Method for Unobtrusive Labeling of Lipopolysaccharides with Quantum Dots

    5. No Access

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      Pages 123-133

      Fluorescence-Based Methods to Assay Inhibitors of Lipopolysaccharide Synthesis

    6. No Access

      Book Protocol

      Pages 135-146

      Micromethods for Lipid A Isolation and Structural Characterization

    7. No Access

      Book Protocol

      Pages 147-160

      Two Efficient Methods for the Conjugation of Smooth-Form Lipopolysaccharides with Probes Bearing Hydrazine or Amino Groups. I. LPS Activation with Cyanogen Bromide

    8. No Access

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      Pages 161-167

      Two Efficient Methods for the Conjugation of Smooth-Form Lipopolysaccharides with Probes Bearing Hydrazine or Amino Groups. II. LPS Activation with a Cyanopyridinium Agent

  4. Mold Fungus Toxins

    1. Front Matter

      Pages 169-169

    2. No Access

      Book Protocol

      Pages 171-185

      Extraction and Analysis of Fumonisins and Compounds Indicative of Fumonisin Exposure in Plant and Mammalian Tissues and Cultured Cells

    3. No Access

      Book Protocol

      Pages 187-194

      Determination of Fumonisins B1 and B2 in Maize Food Products by a New Analytical Method Based on High-Performance Liquid Chromatography and Fluorimetric Detection with Post-column Derivatization

    4. No Access

      Book Protocol

      Pages 195-202

      A Confirmatory Method for Aflatoxin M1 Determination in Milk Based on Immunoaffinity Cleanup and High-Performance Liquid Chromatography with Fluorometric Detection

    5. No Access

      Book Protocol

      Pages 203-210

      Simultaneous Determination of Aflatoxins B1, B2, G1, and G2 in Foods and Feed Materials

    6. No Access

      Book Protocol

      Pages 211-216

      Highly Sensitive PCR-Based Detection Specific to Aspergillus flavus

    7. No Access

      Book Protocol

      Pages 217-235

      A Rapid Enzymatic Method for Aflatoxin B Detection

  5. Back Matter

    Pages 237-239