Viral Vectors for Gene Therapy

Methods and Protocols

Editors:

ISBN: 978-1-61779-094-2 (Print) 978-1-61779-095-9 (Online)

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi

  2. No Access

    Book Protocol

    Pages 1-25

    Introduction to Viral Vectors

  3. No Access

    Book Protocol

    Pages 27-44

    Introduction to Gene Therapy: A Clinical Aftermath

  4. No Access

    Book Protocol

    Pages 45-88

    Host Cells and Cell Banking

  5. No Access

    Book Protocol

    Pages 89-116

    Overview of Current Scalable Methods for Purification of Viral Vectors

  6. No Access

    Book Protocol

    Pages 117-138

    Methods to Construct Recombinant Adenovirus Vectors

  7. No Access

    Book Protocol

    Pages 139-156

    Manufacturing of Adenovirus Vectors: Production and Purification of Helper Dependent Adenovirus

  8. No Access

    Book Protocol

    Pages 157-182

    Manufacturing of Retroviruses

  9. No Access

    Book Protocol

    Pages 183-209

    Lentiviral Vectors

  10. No Access

    Book Protocol

    Pages 211-234

    Adeno-Associated Viruses

  11. No Access

    Book Protocol

    Pages 235-246

    Manufacturing of Adeno-Associated Viruses, for Example: AAV2

  12. No Access

    Book Protocol

    Pages 247-278

    Vector Characterization Methods for Quality Control Testing of Recombinant Adeno-Associated Viruses

  13. No Access

    Book Protocol

    Pages 279-301

    Baculoviruses Mediate Efficient Gene Expression in a Wide Range of Vertebrate Cells

  14. No Access

    Book Protocol

    Pages 303-343

    Herpes Simplex Virus Type 1-Derived Recombinant and Amplicon Vectors

  15. No Access

    Book Protocol

    Pages 345-366

    Manufacture of Measles Viruses

  16. No Access

    Book Protocol

    Pages 367-390

    In Vivo Gene Delivery into hCD34+ Cells in a Humanized Mouse Model

  17. No Access

    Book Protocol

    Pages 391-405

    In Vivo Evaluation of Gene Transfer into Mesenchymal Cells (In View of Cartilage Repair)

  18. No Access

    Book Protocol

    Pages 407-424

    Ethical Consideration

  19. No Access

    Book Protocol

    Pages 425-442

    Clinical Trials of GMP Products in the Gene Therapy Field

  20. Back Matter

    Pages 443-450