MicroRNAs in Development

Methods and Protocols

Editors:

ISBN: 978-1-61779-082-9 (Print) 978-1-61779-083-6 (Online)

Table of contents (15 protocols)

  1. Front Matter

    Pages i-x

  2. No Access

    Book Protocol

    Pages 1-8

    In Situ Detection of microRNAs in Animals

  3. No Access

    Book Protocol

    Pages 9-23

    Detection of microRNAs in Plants by In Situ Hybridisation

  4. No Access

    Book Protocol

    Pages 25-38

    Detecting sRNAs by Northern Blotting

  5. No Access

    Book Protocol

    Pages 39-54

    Profiling MicroRNAs by Real-Time PCR

  6. No Access

    Book Protocol

    Pages 55-68

    Detection of Small RNAs and MicroRNAs Using Deep Sequencing Technology

  7. No Access

    Book Protocol

    Pages 69-88

    Detection of MicroRNAs in Prostate Cancer Cells by MicroRNA Array

  8. No Access

    Book Protocol

    Pages 89-97

    MicroRNA Knock Down by Cholesterol-Conjugated Antisense Oligos in Mouse Organ Culture

  9. No Access

    Book Protocol

    Pages 99-120

    Protocols for Use of Homologous Recombination Gene Targeting to Produce MicroRNA Mutants in Drosophila

  10. No Access

    Book Protocol

    Pages 121-130

    Engineering Elements for Gene Silencing: The Artificial MicroRNAs Technology

  11. No Access

    Book Protocol

    Pages 131-137

    Mimicry Technology: Suppressing Small RNA Activity in Plants

  12. No Access

    Book Protocol

    Pages 139-152

    Experimental Validation of MicroRNA Targets Using a Luciferase Reporter System

  13. No Access

    Book Protocol

    Pages 153-167

    Experimental Identification of MicroRNA Targets by Immunoprecipitation of Argonaute Protein Complexes

  14. No Access

    Book Protocol

    Pages 169-185

    Comprehensive Identification of miRNA Target Sites in Live Animals

  15. No Access

    Book Protocol

    Pages 187-208

    Target Validation of Plant microRNAs

  16. No Access

    Book Protocol

    Pages 209-223

    A High-Throughput Sequencing-Based Methodology to Identify All Uncapped and Cleaved RNA Molecules in Eukaryotic Genomes

  17. Back Matter

    Pages 225-227